دورية أكاديمية
No association between the -308 polymorphism in the tumour necrosis factor alpha (TNFalpha) promoter region and polycystic ovaries.
| العنوان: | No association between the -308 polymorphism in the tumour necrosis factor alpha (TNFalpha) promoter region and polycystic ovaries. |
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| المؤلفون: | Milner CR; Reproductive Medicine Unit, The University of Adelaide, The Queen Elizabeth Hospital, Woodville, South Australia, Australia., Craig JE, Hussey ND, Norman RJ |
| المصدر: | Molecular human reproduction [Mol Hum Reprod] 1999 Jan; Vol. 5 (1), pp. 5-9. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press for the European Society for Human Reproduction and Embryology Country of Publication: England NLM ID: 9513710 Publication Model: Print Cited Medium: Print ISSN: 1360-9947 (Print) Linking ISSN: 13609947 NLM ISO Abbreviation: Mol Hum Reprod Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Oxford University Press for the European Society for Human Reproduction and Embryology Original Publication: Oxford, UK : Published for the European Society for Human Reproduction and Embryology by Oxford University Press, [1995- |
| مواضيع طبية MeSH: | Polymorphism, Genetic*, Polycystic Ovary Syndrome/*genetics , Tumor Necrosis Factor-alpha/*genetics, Adult ; Female ; Glucose Tolerance Test ; Heterozygote ; Humans ; Insulin Resistance/genetics ; Menstrual Cycle/physiology ; Obesity/genetics ; Ovulation/genetics ; Polycystic Ovary Syndrome/diagnostic imaging ; Polymorphism, Single-Stranded Conformational ; Promoter Regions, Genetic ; Ultrasonography |
| مستخلص: | The tumour necrosis factor (TNF)2 allele appears to be linked with increased insulin resistance and obesity, conditions often found in overweight patients with polycystic ovary syndrome (PCOS). The significance of TNFalpha polymorphism in relation to the clinical and biochemical parameters associated with PCOS was investigated in 122 well-characterized patients with polycystic ovaries (PCO). Of these, 84 had an abnormal menstrual cycle and were classified as having PCOS, while the remaining 38 had a normal menstrual cycle and were classified as having PCO. There were a further 28 individuals without PCO (non-PCO) and 108 individuals whose PCO status was undetermined (reference population). The promoter region of the TNFalpha gene was amplified by polymerase chain reaction (PCR), and the presence or absence of the polymorphism at -308 was determined by single-strand conformational polymorphism (SSCP) analysis. The less common TNF allele (TNF2) was found as TNF1/2 or TNF2/2 in 11/38 (29%) of PCO subjects, 25/84 (30%) of PCOS subjects, 7/28 (25%) of non-PCO subjects, and 45/108 (42%) of the reference population. There was no significant difference in the incidence of the TNF2 allele between the groups. The relationship of TNF genotype to clinical and biochemical parameters was examined. In both the PCO group and the PCOS group, the presence of the TNF2 allele was significantly associated with lower glucose values obtained from the glucose tolerance testing (P<0.05). The TNF genotype was not significantly associated with any clinical or biochemical parameter measured in the PCO, PCOS or non-PCOS groups. Thus, the TNFalpha -308 polymorphism does not appear to strongly influence genetic susceptibility to polycystic ovaries. |
| المشرفين على المادة: | 0 (Tumor Necrosis Factor-alpha) |
| تواريخ الأحداث: | Date Created: 19990302 Date Completed: 19990506 Latest Revision: 20190513 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/molehr/5.1.5 |
| PMID: | 10050654 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1360-9947 |
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| DOI: | 10.1093/molehr/5.1.5 |