دورية أكاديمية
Hydrocarbon chain packing and the effect of ethanol on the thermotropic phase behavior of mixed-chain phosphatidylglycerols.
العنوان: | Hydrocarbon chain packing and the effect of ethanol on the thermotropic phase behavior of mixed-chain phosphatidylglycerols. |
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المؤلفون: | Durvasula RV; Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Box 440, Charlottesville, VA 22908, USA., Huang CH |
المصدر: | Biochimica et biophysica acta [Biochim Biophys Acta] 1999 Feb 04; Vol. 1417 (1), pp. 101-10. |
نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Pub. Co Country of Publication: Netherlands NLM ID: 0217513 Publication Model: Print Cited Medium: Print ISSN: 0006-3002 (Print) Linking ISSN: 00063002 NLM ISO Abbreviation: Biochim Biophys Acta Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier Pub. Co. |
مواضيع طبية MeSH: | Ethanol/*chemistry , Hydrocarbons/*chemistry , Phosphatidylglycerols/*chemistry, Calorimetry, Differential Scanning ; Lipid Bilayers/chemistry ; Models, Chemical ; Molecular Conformation ; Thermodynamics |
مستخلص: | Previous studies in this laboratory have delineated the relationship between the acyl chain asymmetry of mixed-chain phosphatidylcholines and the effect of ethanol concentration ([EtOH]) on their melting behavior (Li et al., Biophys J., 70 (1996) 2784-2794). This present investigation extends these findings to another phospholipid family by using high-resolution differential scanning calorimetry (DSC) to characterize the effect of ethanol concentration on the main phase transition temperature (Tm) of five molecular species of mixed-chain phosphatidylglycerol (PG). For C(14):C(18)PG, C(15):C(17)PG, C(16):C(16)PG, and C(17):C(15)PG, a biphasic profile in the Tm versus [EtOH] plot was observed, and the minimum in the plot for each PG occurred at 33, 15, 19, and 36 mg/ml, respectively. This biphasic behavior is typical of phospholipids whose acyl chain asymmetry is fairly small. For C(18):C(14)PG, only a linear decrease in the Tm was observed as a function of ethanol concentration; this effect is characteristic of highly asymmetric phospholipids. Our DSC results obtained with mixed-chain PG in the presence of ethanol demonstrate that the acyl chain asymmetry of the five lipids studied can be ranked as follows: C(15):C(17)PG |
معلومات مُعتمدة: | GM-08323 United States GM NIGMS NIH HHS; GM-17452 United States GM NIGMS NIH HHS |
المشرفين على المادة: | 0 (Hydrocarbons) 0 (Lipid Bilayers) 0 (Phosphatidylglycerols) 3K9958V90M (Ethanol) |
تواريخ الأحداث: | Date Created: 19990317 Date Completed: 19990329 Latest Revision: 20190610 |
رمز التحديث: | 20240627 |
DOI: | 10.1016/s0005-2736(98)00242-9 |
PMID: | 10076039 |
قاعدة البيانات: | MEDLINE |
تدمد: | 0006-3002 |
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DOI: | 10.1016/s0005-2736(98)00242-9 |