دورية أكاديمية
Molecular identification of the apical Ca2+ channel in 1, 25-dihydroxyvitamin D3-responsive epithelia.
| العنوان: | Molecular identification of the apical Ca2+ channel in 1, 25-dihydroxyvitamin D3-responsive epithelia. |
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| المؤلفون: | Hoenderop JG; Department of Cell Physiology, Institute of Cellular Signaling, University of Nijmegen, P. O. Box 9101, 6500 HB Nijmegen, The Netherlands., van der Kemp AW, Hartog A, van de Graaf SF, van Os CH, Willems PH, Bindels RJ |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 1999 Mar 26; Vol. 274 (13), pp. 8375-8. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print Cited Medium: Print ISSN: 0021-9258 (Print) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Calcitriol/*pharmacology , Calcium Channels/*chemistry , Epithelial Cells/*metabolism , Kidney Tubules/*metabolism, Amino Acid Sequence ; Animals ; Biological Transport ; Calbindins ; Calcium/metabolism ; Calcium Channels/analysis ; Calcium Channels/genetics ; Cells, Cultured ; Cloning, Molecular ; Immunohistochemistry ; Molecular Sequence Data ; RNA, Messenger/metabolism ; Rabbits ; S100 Calcium Binding Protein G/metabolism ; Sequence Alignment ; Sequence Analysis, DNA ; Substrate Specificity ; TRPV Cation Channels |
| مستخلص: | In mammals, the extracellular calcium concentration is maintained within a narrow range despite large variations in daily dietary input and body demand. The small intestine and kidney constitute the influx pathways into the extracellular Ca2+ pool and, therefore, play a primary role in Ca2+ homeostasis. We identified an apical Ca2+ influx channel, which is expressed in proximal small intestine, the distal part of the nephron and placenta. This novel epithelial Ca2+ channel (ECaC) of 730 amino acids contains six putative membrane-spanning domains with an additional hydrophobic stretch predicted to be the pore region. ECaC resembles the recently cloned capsaicin receptor and the transient receptor potential-related ion channels with respect to its predicted topology but shares less than 30% sequence homology with these channels. In kidney, ECaC is abundantly present in the apical membrane of Ca2+ transporting cells and colocalizes with 1,25-dihydroxyvitamin D3-dependent calbindin-D28K. ECaC expression in Xenopus oocytes confers Ca2+ influx with properties identical to those observed in distal renal cells. Thus, ECaC has the expected properties for being the gatekeeper of 1,25-dihydroxyvitamin D3-dependent active transepithelial Ca2+ transport. |
| سلسلة جزيئية: | GENBANK AJ133128 |
| المشرفين على المادة: | 0 (Calbindins) 0 (Calcium Channels) 0 (ECaC protein, Oryctolagus cuniculus) 0 (RNA, Messenger) 0 (S100 Calcium Binding Protein G) 0 (TRPV Cation Channels) FXC9231JVH (Calcitriol) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 19990320 Date Completed: 19990429 Latest Revision: 20210209 |
| رمز التحديث: | 20240513 |
| DOI: | 10.1074/jbc.274.13.8375 |
| PMID: | 10085067 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0021-9258 |
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| DOI: | 10.1074/jbc.274.13.8375 |