دورية أكاديمية
Production and characterization of anti-peptide monoclonal antibodies with specificity for staphylococcal enterotoxins A and B.
| العنوان: | Production and characterization of anti-peptide monoclonal antibodies with specificity for staphylococcal enterotoxins A and B. |
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| المؤلفون: | Bhatti AR; Defence Research Establishment Suffield, Ralston, AB, Canada., Micusan VV |
| المصدر: | Journal of microbiological methods [J Microbiol Methods] 1999 Mar; Vol. 35 (2), pp. 143-9. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print Cited Medium: Print ISSN: 0167-7012 (Print) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, The Netherlands : Elsevier Biomedical, c1983- |
| مواضيع طبية MeSH: | Antibodies, Bacterial/*biosynthesis , Antibodies, Monoclonal/*biosynthesis , Enterotoxins/*immunology , Peptides/*immunology , Staphylococcus aureus/*immunology, Animals ; Antibodies, Bacterial/immunology ; Antibodies, Monoclonal/immunology ; Antibody Specificity ; Enterotoxins/biosynthesis ; Enzyme-Linked Immunosorbent Assay ; Female ; Mice ; Mice, Inbred BALB C ; Peptides/chemical synthesis ; Rabbits ; Staphylococcus aureus/metabolism |
| مستخلص: | A synthetic peptide containing selected epitopes from staphylococcal enterotoxin A (SEA) and enterotoxin B (SEB) was used to produce monoclonal antibodies (Mabs) to respective enterotoxins in a single fusion procedure. The peptide inhibited the reaction of polyclonal anti-SEA or anti-SEB antisera with their homologous enterotoxin, thus showing that the chosen epitopes are part of the antibody-inducing enterotoxin sequences. Two Mabs, Mab-A and Mab-B, reacted with both the peptide and with either SEA or SEB. Used in a double antibody sandwich ELISA, the Mabs were able to quantitate the native SEA or SEB toxins at nanogram levels. |
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| المشرفين على المادة: | 0 (Antibodies, Bacterial) 0 (Antibodies, Monoclonal) 0 (Enterotoxins) 0 (Peptides) 37337-57-8 (enterotoxin A, Staphylococcal) 39424-53-8 (enterotoxin B, staphylococcal) |
| تواريخ الأحداث: | Date Created: 19990407 Date Completed: 19990706 Latest Revision: 20210119 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC7133826 |
| DOI: | 10.1016/s0167-7012(98)00110-9 |
| PMID: | 10192046 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0167-7012 |
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| DOI: | 10.1016/s0167-7012(98)00110-9 |