دورية أكاديمية
An early S phase checkpoint is regulated by the E2F1 transcription factor.
| العنوان: | An early S phase checkpoint is regulated by the E2F1 transcription factor. |
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| المؤلفون: | Stubbs MC; Deptartment of Orthopaedic Surgery, Thomas Jefferson University, Room 501 Curtis Building, Philadelphia, Pennsylvania, 19107, USA., Strachan GD, Hall DJ |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1999 Apr 29; Vol. 258 (1), pp. 77-80. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Carrier Proteins* , Cell Cycle Proteins* , DNA-Binding Proteins*, S Phase/*physiology , Transcription Factors/*physiology, 3T3 Cells ; Animals ; DNA/metabolism ; E2F Transcription Factors ; E2F1 Transcription Factor ; Mice ; Retinoblastoma-Binding Protein 1 ; Thymidine/metabolism ; Transcription Factor DP1 |
| مستخلص: | The E2F1 transcription factor regulates transit of cells through the S phase checkpoint, dependent on its association with cyclin A/cdk2. Expression in cells of a mutant E2F1 lacking the cyclin A/cdk2 binding domain leads to partial arrest of cells at the S phase checkpoint. When subconfluent growing cells expressing this mutant E2F1 are analyzed in detail, it is shown here that they display a significantly reduced incorporation of 3H-thymidine into the DNA of each S phase cell, compared to control cells or to cells overexpressing full-length E2F1. Further, when cells are blocked at the G1/S phase border and released, there is a clear reduction in the amount of 3H-thymidine incorporated into the DNA of S phase cells by 1.5 hours post release. Considering a normal 6 hour S phase duration, the results show that the S phase checkpoint mediated by E2F1 is not a late S phase event but an early one. (Copyright 1999 Academic Press.) |
| معلومات مُعتمدة: | CA67032 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (Arid4a protein, mouse) 0 (Carrier Proteins) 0 (Cell Cycle Proteins) 0 (DNA-Binding Proteins) 0 (E2F Transcription Factors) 0 (E2F1 Transcription Factor) 0 (E2f1 protein, mouse) 0 (Retinoblastoma-Binding Protein 1) 0 (Transcription Factor DP1) 0 (Transcription Factors) 9007-49-2 (DNA) VC2W18DGKR (Thymidine) |
| تواريخ الأحداث: | Date Created: 19990501 Date Completed: 19990527 Latest Revision: 20131121 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1006/bbrc.1999.0603 |
| PMID: | 10222238 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0006-291X |
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| DOI: | 10.1006/bbrc.1999.0603 |