دورية أكاديمية
IL-2-deficient mice raised under germfree conditions develop delayed mild focal intestinal inflammation.
| العنوان: | IL-2-deficient mice raised under germfree conditions develop delayed mild focal intestinal inflammation. |
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| المؤلفون: | Schultz M; Center for Gastrointestinal Biology and Disease, Department of Medicine, University of North Carolina, Chapel Hill, NC 27599, USA., Tonkonogy SL, Sellon RK, Veltkamp C, Godfrey VL, Kwon J, Grenther WB, Balish E, Horak I, Sartor RB |
| المصدر: | The American journal of physiology [Am J Physiol] 1999 Jun; Vol. 276 (6), pp. G1461-72. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 0370511 Publication Model: Print Cited Medium: Print ISSN: 0002-9513 (Print) Linking ISSN: 00029513 NLM ISO Abbreviation: Am J Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Bethesda, MD : American Physiological Society Original Publication: Washington [etc.] American Physiological Society. |
| مواضيع طبية MeSH: | Colitis/*etiology , Gastritis/*etiology , Interleukin-2/*deficiency, Animals ; Colitis/pathology ; Colon/metabolism ; Digestive System/pathology ; Gastritis/pathology ; Germ-Free Life ; Hepatitis, Animal/etiology ; Immunoglobulins/biosynthesis ; Interleukin-2/genetics ; Interleukin-2/metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Knockout/genetics ; Time Factors |
| مستخلص: | Interleukin-2 (IL-2) amplifies immune stimuli and influences B cell differentiation. IL-2-deficient mice spontaneously develop intestinal inflammation if raised under specific pathogen-free (SPF) conditions. We quantitatively determined the aggressiveness and kinetics of gastrointestinal and hepatic inflammation in the presence or absence of viable bacteria in IL-2-deficient mice. Breeding colonies were maintained under SPF and germfree (GF) conditions. Intestinal tissues, serum, and mesenteric lymph nodes were obtained from mice at different ages for blind histological scoring, immunoglobulin measurements, mucosal T cell infiltration, and cytokine secretion. GF IL-2 -/- mice developed mild, focal, and nonlethal intestinal inflammation with delayed onset, whereas the more aggressive inflammation in SPF IL-2 -/- mice led to their death between 28 and 32 wk. Periportal hepatic inflammation was equal in the presence or absence of bacterial colonization. Intestinal immunoglobulin secretion decreased significantly by 13 wk of age in IL-2 -/- mice in both GF and SPF environments. In contrast to other genetically engineered rodents, IL-2 -/- mice develop mild focal gastrointestinal and active portal tract inflammation in the absence of viable bacteria. |
| معلومات مُعتمدة: | DK-34087 United States DK NIDDK NIH HHS; DK-40249 United States DK NIDDK NIH HHS; DK-53347 United States DK NIDDK NIH HHS |
| المشرفين على المادة: | 0 (Immunoglobulins) 0 (Interleukin-2) |
| تواريخ الأحداث: | Date Created: 19990611 Date Completed: 19990729 Latest Revision: 20181218 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1152/ajpgi.1999.276.6.G1461 |
| PMID: | 10362650 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0002-9513 |
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| DOI: | 10.1152/ajpgi.1999.276.6.G1461 |