دورية أكاديمية
New substrates of DNA polymerases.
| العنوان: | New substrates of DNA polymerases. |
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| المؤلفون: | Victorova L; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow., Sosunov V, Skoblov A, Shipytsin A, Krayevsky A |
| المصدر: | FEBS letters [FEBS Lett] 1999 Jun 18; Vol. 453 (1-2), pp. 6-10. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 0155157 Publication Model: Print Cited Medium: Print ISSN: 0014-5793 (Print) Linking ISSN: 00145793 NLM ISO Abbreviation: FEBS Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd. Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies. |
| مواضيع طبية MeSH: | DNA-Directed DNA Polymerase/*metabolism , Dinucleoside Phosphates/*chemistry , Dinucleoside Phosphates/*metabolism, Bacteria/enzymology ; DNA Polymerase I/metabolism ; Humans ; RNA-Directed DNA Polymerase/metabolism ; Substrate Specificity ; Taq Polymerase/metabolism ; Viruses/enzymology |
| مستخلص: | Bis-(2'-deoxynucleoside) 5',5'-tetraphosphates and bis-(2'-deoxynucleoside) 5',5'-triphosphates were shown to be a new type of substrate for several DNA polymerases of human, bacterial and viral origin. Their substrate properties depend both on their structure and on the nature of the enzyme. They are incorporated by both termini in correspondence with the template nucleotide program in the active center. The results obtained support the mechanism of their direct incorporation rather than prior hydrolysis to dNTP. The highest activity of these compounds was observed for HIV reverse transcriptase. The probable biological significance of the reaction is discussed. |
| المشرفين على المادة: | 0 (Dinucleoside Phosphates) EC 2.7.7.- (Taq Polymerase) EC 2.7.7.49 (RNA-Directed DNA Polymerase) EC 2.7.7.7 (DNA Polymerase I) EC 2.7.7.7 (DNA-Directed DNA Polymerase) |
| تواريخ الأحداث: | Date Created: 19990714 Date Completed: 19990802 Latest Revision: 20201209 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/s0014-5793(99)00615-8 |
| PMID: | 10403364 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0014-5793 |
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| DOI: | 10.1016/s0014-5793(99)00615-8 |