دورية أكاديمية
Expression of pTalpha mRNA in a committed dendritic cell precursor in the human thymus.
| العنوان: | Expression of pTalpha mRNA in a committed dendritic cell precursor in the human thymus. |
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| المؤلفون: | Res PC; Division of Immunology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Huis, Amsterdam, The Netherlands., Couwenberg F, Vyth-Dreese FA, Spits H |
| المصدر: | Blood [Blood] 1999 Oct 15; Vol. 94 (8), pp. 2647-57. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 7603509 Publication Model: Print Cited Medium: Print ISSN: 0006-4971 (Print) Linking ISSN: 00064971 NLM ISO Abbreviation: Blood Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York] : Elsevier Original Publication: New York, Grune & Stratton [etc.] |
| مواضيع طبية MeSH: | Gene Expression Regulation, Developmental*, Dendritic Cells/*cytology , Membrane Glycoproteins/*biosynthesis , Stem Cells/*cytology , Thymus Gland/*cytology, Animals ; Antigens, CD/analysis ; CD40 Ligand ; Cell Differentiation/drug effects ; Cell Lineage ; Child, Preschool ; Coculture Techniques ; Homeodomain Proteins/biosynthesis ; Homeodomain Proteins/genetics ; Humans ; Infant ; Infant, Newborn ; Interleukin-3/pharmacology ; Lymphoid Tissue/cytology ; Membrane Glycoproteins/genetics ; Membrane Glycoproteins/pharmacology ; Mice ; Mice, Knockout ; Microscopy, Confocal ; Organ Specificity ; Palatine Tonsil/cytology ; RNA, Messenger/biosynthesis ; RNA, Messenger/genetics ; Receptors, Antigen, T-Cell, alpha-beta ; Reverse Transcriptase Polymerase Chain Reaction ; Stem Cells/drug effects ; Stem Cells/metabolism |
| مستخلص: | We have characterized dendritic cell precursors (pre-DC) in the human thymus. These CD1a(-)CD3(-)CD4(+)CD8(-) cells express high levels of interleukin-3Ralpha (IL-3Ralpha) on the membrane and are able to develop into mature DC upon culture with IL-3 and CD40 ligation. The DC precursors are predominantly located in the thymic medulla. Interestingly, the pre-DC express pTalpha mRNA, which is also present in CD1a(+)CD3(-)CD4(+)CD8(-) pre-T cells. Yet, the pre-DC lack expression of recombination activating gene-1 mRNA and fail to develop into T cells in appropriate assays. The thymic pre-DC are very similar to the recently characterized pre-DC found in the T cell areas of the tonsil, and it is suggested that these pre-DC populations are of lymphoid origin. |
| المشرفين على المادة: | 0 (Antigens, CD) 0 (Homeodomain Proteins) 0 (Interleukin-3) 0 (Membrane Glycoproteins) 0 (RNA, Messenger) 0 (Receptors, Antigen, T-Cell, alpha-beta) 0 (pre-T cell receptor alpha) 128559-51-3 (RAG-1 protein) 147205-72-9 (CD40 Ligand) |
| تواريخ الأحداث: | Date Created: 19991009 Date Completed: 19991122 Latest Revision: 20210216 |
| رمز التحديث: | 20221213 |
| PMID: | 10515868 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0006-4971 |
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