دورية أكاديمية
Increased sensitivity to endotoxemia in the bile duct-ligated cirrhotic Rat.
| العنوان: | Increased sensitivity to endotoxemia in the bile duct-ligated cirrhotic Rat. |
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| المؤلفون: | Harry D; Department of Medicine, Royal Free and University College Hospital School of Medicine, Royal Free Campus, Pond Street, London, UK., Anand R, Holt S, Davies S, Marley R, Fernando B, Goodier D, Moore K |
| المصدر: | Hepatology (Baltimore, Md.) [Hepatology] 1999 Nov; Vol. 30 (5), pp. 1198-205. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wolters Kluwer Health, Inc Country of Publication: United States NLM ID: 8302946 Publication Model: Print Cited Medium: Print ISSN: 0270-9139 (Print) Linking ISSN: 02709139 NLM ISO Abbreviation: Hepatology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [Philadelphia] : Wolters Kluwer Health, Inc. Original Publication: Baltimore, MD : Williams & Wilkins, [c1981]- |
| مواضيع طبية MeSH: | Lipid Peroxidation*, Endotoxemia/*physiopathology , Lipopolysaccharides/*toxicity , Liver/*pathology , Liver Cirrhosis, Experimental/*physiopathology , Tumor Necrosis Factor-alpha/*metabolism, Animals ; Bile Ducts/physiology ; Cell Nucleus/metabolism ; Dinoprost/blood ; Disease Susceptibility ; Endotoxemia/blood ; Endotoxemia/chemically induced ; Kidney Function Tests ; Liver/drug effects ; Liver/physiopathology ; Liver Cirrhosis, Experimental/blood ; Liver Cirrhosis, Experimental/pathology ; Liver Function Tests ; Male ; NF-kappa B/metabolism ; Nitrates/blood ; Nitrites/blood ; Rats ; Rats, Sprague-Dawley ; Tumor Necrosis Factor-alpha/analysis |
| مستخلص: | Sepsis is a common complication of cirrhosis with a high mortality. In this study, we have investigated some of the pathways that may be involved in tissue injury and death. Bile duct-ligated (BDL) cirrhotic and control rats were challenged with lipopolysaccharide (LPS). Sensitivity to LPS was markedly enhanced in the BDL group, and was associated with increased liver injury and mortality. There was a 5-fold constitutive activation of nuclear factor kappa B (NFkappaB) in the liver of BDL rat controls (P <.001), and this was activated further, but to a similar extent, in the liver of both sham and BDL rats after injection of LPS. Plasma tumor necrosis factor alpha (TNF-alpha) increased more markedly in the BDL cirrhotic rats (2,463 +/- 697 pg/mL in BDL rats versus 401 +/- 160 pg/mL in the controls at 3 hours; P <.01). Plasma nitrite/nitrate concentrations were increased in the BDL controls at baseline, and increased further after LPS (P <.05), but did not differ from sham controls at 6 hours. Plasma F(2)-isoprostanes increased 6-fold in the cirrhotic rats and 2-fold in the controls (P <.01) indicative of lipid peroxidation. Esterified F(2)-isoprostanes in the liver increased 2- to 3-fold at 1 hour in control and BDL rats, but returned to baseline levels by 3 hours. Esterified F(2)-isoprostanes in the kidney increased by 2-fold in the BDL rats after LPS administration, but remained unchanged in sham controls. We conclude that there is a marked increase in sensitivity to LPS in BDL cirrhotic rats. This is associated with an enhanced TNF-alpha response and increased lipid peroxidation. These may be directly and causally related to mortality. |
| المشرفين على المادة: | 0 (Lipopolysaccharides) 0 (NF-kappa B) 0 (Nitrates) 0 (Nitrites) 0 (Tumor Necrosis Factor-alpha) B7IN85G1HY (Dinoprost) |
| تواريخ الأحداث: | Date Created: 19991026 Date Completed: 19991124 Latest Revision: 20220311 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/hep.510300515 |
| PMID: | 10534341 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0270-9139 |
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| DOI: | 10.1002/hep.510300515 |