دورية أكاديمية
Acetogenic and sulfate-reducing bacteria inhabiting the rhizoplane and deep cortex cells of the sea grass Halodule wrightii.
| العنوان: | Acetogenic and sulfate-reducing bacteria inhabiting the rhizoplane and deep cortex cells of the sea grass Halodule wrightii. |
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| المؤلفون: | Küsel K; Gulf Ecology Division, U.S. EPA/National Health and Environmental Effects Research Laboratory, Gulf Breeze, Florida 32561, USA., Pinkart HC, Drake HL, Devereux R |
| المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 1999 Nov; Vol. 65 (11), pp. 5117-23. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print Cited Medium: Print ISSN: 0099-2240 (Print) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Acetates/*metabolism , Acetobacteraceae/*physiology , Desulfovibrio/*physiology , Geologic Sediments/*microbiology , Gram-Positive Bacteria/*physiology , Poaceae/*microbiology , Sulfates/*metabolism, Acetobacteraceae/classification ; Acetobacteraceae/isolation & purification ; Desulfovibrio/classification ; Desulfovibrio/isolation & purification ; Gram-Positive Bacteria/classification ; Gram-Positive Bacteria/isolation & purification ; Oligonucleotide Probes ; RNA, Bacterial/genetics ; RNA, Ribosomal, 16S/genetics ; Seawater/microbiology ; Substrate Specificity |
| مستخلص: | Recent declines in sea grass distribution underscore the importance of understanding microbial community structure-function relationships in sea grass rhizospheres that might affect the viability of these plants. Phospholipid fatty acid analyses showed that sulfate-reducing bacteria and clostridia were enriched in sediments colonized by the sea grasses Halodule wrightii and Thalassia testudinum compared to an adjacent unvegetated sediment. Most-probable-number analyses found that in contrast to butyrate-producing clostridia, acetogens and acetate-utilizing sulfate reducers were enriched by an order of magnitude in rhizosphere sediments. Although sea grass roots are oxygenated in the daytime, colorimetric root incubation studies demonstrated that acetogenic O-demethylation and sulfidogenic iron precipitation activities were tightly associated with washed, sediment-free H. wrightii roots. This suggests that the associated anaerobes are able to tolerate exposure to oxygen. To localize and quantify the anaerobic microbial colonization, root thin sections were hybridized with newly developed (33)P-labeled probes that targeted (i) low-G+C-content gram-positive bacteria, (ii) cluster I species of clostridia, (iii) species of Acetobacterium, and (iv) species of Desulfovibrio. Microautoradiography revealed intercellular colonization of the roots by Acetobacterium and Desulfovibrio species. Acetogenic bacteria occurred mostly in the rhizoplane and outermost cortex cell layers, and high numbers of sulfate reducers were detected on all epidermal cells and inward, colonizing some 60% of the deepest cortex cells. Approximately 30% of epidermal cells were colonized by bacteria that hybridized with an archaeal probe, strongly suggesting the presence of methanogens. Obligate anaerobes within the roots might contribute to the vitality of sea grasses and other aquatic plants and to the biogeochemistry of the surrounding sediment. |
| References: | Appl Environ Microbiol. 1995 Jul;61(7):2681-7. (PMID: 16535076) Appl Environ Microbiol. 1995 Mar;61(3):1013-9. (PMID: 16534951) Nucleic Acids Res. 1994 Sep;22(17):3485-7. (PMID: 7524021) Science. 1983 Oct 7;222(4619):51-3. (PMID: 17810089) Int J Syst Bacteriol. 1994 Oct;44(4):812-26. (PMID: 7981107) Appl Environ Microbiol. 1995 Oct;61(10):3667-75. (PMID: 16535147) J Bacteriol. 1996 Jun;178(12):3496-500. (PMID: 8655546) Appl Environ Microbiol. 1992 Feb;58(2):614-23. (PMID: 1376982) Appl Environ Microbiol. 1978 Apr;35(4):762-5. (PMID: 348108) Int J Syst Bacteriol. 1997 Apr;47(2):352-8. (PMID: 9103621) Can J Microbiol. 1980 Aug;26(8):998-1005. (PMID: 6936067) Biofactors. 1997;6(1):13-24. (PMID: 9233536) J Bacteriol. 1990 Feb;172(2):762-70. (PMID: 1688842) Microbiol Rev. 1995 Mar;59(1):143-69. (PMID: 7535888) Science. 1982 May 7;216(4546):633-5. (PMID: 17783311) Appl Environ Microbiol. 1998 Dec;64(12):4983-9. (PMID: 9835592) Appl Environ Microbiol. 1992 Jul;58(7):2158-63. (PMID: 1379029) Appl Environ Microbiol. 1996 Feb;62(2):494-500. (PMID: 16535237) Appl Environ Microbiol. 1996 Dec;62(12):4504-13. (PMID: 8953722) Science. 1991 Mar 22;251:1471-3. (PMID: 11538266) FEMS Microbiol Lett. 1994 Jun 15;119(3):303-8. (PMID: 7519575) Appl Environ Microbiol. 1994 Apr;60(4):1232-40. (PMID: 7517128) Appl Environ Microbiol. 1994 Sep;60(9):3220-7. (PMID: 7524441) Appl Environ Microbiol. 1992 Jan;58(1):70-7. (PMID: 16348641) Appl Environ Microbiol. 1997 Jan;63(1):296-300. (PMID: 16535493) Nucleic Acids Res. 1994 Nov 11;22(22):4673-80. (PMID: 7984417) |
| المشرفين على المادة: | 0 (Acetates) 0 (Oligonucleotide Probes) 0 (RNA, Bacterial) 0 (RNA, Ribosomal, 16S) 0 (Sulfates) |
| تواريخ الأحداث: | Date Created: 19991105 Date Completed: 19991213 Latest Revision: 20200724 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC91688 |
| DOI: | 10.1128/AEM.65.11.5117-5123.1999 |
| PMID: | 10543830 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0099-2240 |
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| DOI: | 10.1128/AEM.65.11.5117-5123.1999 |