دورية أكاديمية
Oral anti-IgE immunization with epitope-displaying phage.
| العنوان: | Oral anti-IgE immunization with epitope-displaying phage. |
|---|---|
| المؤلفون: | Zuercher AW; Institute of Immunology, University of Bern, Inselspital, Bern, Switzerland., Miescher SM, Vogel M, Rudolf MP, Stadler MB, Stadler BM |
| المصدر: | European journal of immunology [Eur J Immunol] 2000 Jan; Vol. 30 (1), pp. 128-35. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 1273201 Publication Model: Print Cited Medium: Print ISSN: 0014-2980 (Print) Linking ISSN: 00142980 NLM ISO Abbreviation: Eur J Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2005->: Weinheim : Wiley-VCH Original Publication: Weinheim, Verlag Chemie GmbH. |
| مواضيع طبية MeSH: | Bacteriophages/*genetics , Immunoglobulin E/*immunology , Vaccines/*administration & dosage, Administration, Oral ; Animals ; Antibodies, Anti-Idiotypic/blood ; Antibodies, Monoclonal/immunology ; Antibody Specificity ; Epitopes ; Female ; Immunization ; Immunoglobulin Fab Fragments/immunology ; Mice ; Mice, Inbred BALB C ; Polymerase Chain Reaction |
| مستخلص: | An essential requirement for oral vaccines is the ability to survive the harsh environment of the stomach in an antigenically intact form. As bacteriophages are adapted to this environment we used epitope-displaying M13 bacteriophages as carriers for an experimental oral anti-IgE vaccine. The feasibility of this approach was tested in a simulated gastric fluid using two different mimotopes as well as an anti-idiotypic Fab of the non-anaphylactogenic monoclonal anti-IgE antibody BSW17. All phage clones remained infective after this treatment. However, only epitopes displayed on the pVIII protein were still recognized by BSW17 whereas pIII-expressed epitopes were rapidly inactivated. Surprisingly, when used for oral immunization of mice all phage clones induced anti-IgE antibodies. In contrast, oral immunization with the purified, pVIII protein displaying the mimotope induced anti-phage but no anti-IgE antibodies. After feeding a single dose of mimotope-displaying bacteriophage, phage DNA could be detected in mouse feces for 10 days. Our results show that epitope-displaying bacteriophages can be used to induce an epitope-specific antibody response via the oral route. |
| المشرفين على المادة: | 0 (Antibodies, Anti-Idiotypic) 0 (Antibodies, Monoclonal) 0 (Epitopes) 0 (Immunoglobulin Fab Fragments) 0 (Vaccines) 0 (anti-IgE antibodies) 37341-29-0 (Immunoglobulin E) |
| تواريخ الأحداث: | Date Created: 19991222 Date Completed: 20000124 Latest Revision: 20130118 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/1521-4141(200001)30:1<128::AID-IMMU128>3.0.CO;2-X |
| PMID: | 10602034 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0014-2980 |
|---|---|
| DOI: | 10.1002/1521-4141(200001)30:1<128::AID-IMMU128>3.0.CO;2-X |