دورية أكاديمية
Use of polymerase chain reaction to diagnose the fifth reported US case of autochthonous transmission of Trypanosoma cruzi, in Tennessee, 1998.
| العنوان: | Use of polymerase chain reaction to diagnose the fifth reported US case of autochthonous transmission of Trypanosoma cruzi, in Tennessee, 1998. |
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| المؤلفون: | Herwaldt BL; Centers for Disease Control and Prevention, Division of Parasitic Diseases, Mailstop F22, 4770 Buford Hwy. N.E., Atlanta, GA 30341-3724, USA. bxh4@cdc.gov, Grijalva MJ, Newsome AL, McGhee CR, Powell MR, Nemec DG, Steurer FJ, Eberhard ML |
| المصدر: | The Journal of infectious diseases [J Infect Dis] 2000 Jan; Vol. 181 (1), pp. 395-9. |
| نوع المنشور: | Case Reports; Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0413675 Publication Model: Print Cited Medium: Print ISSN: 0022-1899 (Print) Linking ISSN: 00221899 NLM ISO Abbreviation: J Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2011- : Oxford : Oxford University Press Original Publication: 1904-2010 : Chicago, IL : University of Chicago Press |
| مواضيع طبية MeSH: | Polymerase Chain Reaction*, Chagas Disease/*diagnosis , Triatoma/*parasitology, Animals ; Chagas Disease/transmission ; DNA, Protozoan/blood ; Female ; Humans ; Infant, Newborn ; Insect Vectors/parasitology ; Intestines/parasitology ; Male ; Parasitemia/diagnosis ; Tennessee |
| مستخلص: | In July 1998, the mother of an 18-month-old boy in rural Tennessee found a triatomine bug in his crib, which she saved because it resembled a bug shown on a television program about insects that prey on mammals. The gut contents of the Triatoma sanguisuga were found, by light microscopy and polymerase chain reaction (PCR), to be infected with Trypanosoma cruzi; PCR products hybridized with T. cruzi-specific oligonucleotide probes. Whole-blood specimens obtained from the child in July and August were negative by buffy-coat examination and hemoculture but positive by PCR and DNA hybridization, suggesting that he had low-level parasitemia. Specimens obtained after treatment with benznidazole were negative. He did not develop anti-T. cruzi antibody; 19 relatives and neighbors also were seronegative. Two of 3 raccoons trapped in the vicinity had positive hemocultures for T. cruzi. The child's case of T. cruzi infection-the fifth reported US autochthonous case-would have been missed without his mother's attentiveness and the availability of sensitive molecular techniques. |
| Number of References: | 15 |
| المشرفين على المادة: | 0 (DNA, Protozoan) |
| تواريخ الأحداث: | Date Created: 19991223 Date Completed: 20000303 Latest Revision: 20051116 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1086/315212 |
| PMID: | 10608796 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0022-1899 |
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| DOI: | 10.1086/315212 |