دورية أكاديمية
أعرض في EDS

Neural regulation of phenylethanolamine N-methyltransferase (PNMT) gene expression in bovine chromaffin cells differs from other catecholamine enzyme genes.

التفاصيل البيبلوغرافية
العنوان: Neural regulation of phenylethanolamine N-methyltransferase (PNMT) gene expression in bovine chromaffin cells differs from other catecholamine enzyme genes.
المؤلفون: Lee YS; Department of Pediatrics, SUNY at Stony Brook, NY 11794-8111, USA., Raia G, Tönshoff C, Evinger MJ
المصدر: Journal of molecular neuroscience : MN [J Mol Neurosci] 1999 Feb; Vol. 12 (1), pp. 53-68.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, P.H.S.
اللغة: English
بيانات الدورية: Publisher: Humana Press Country of Publication: United States NLM ID: 9002991 Publication Model: Print Cited Medium: Print ISSN: 0895-8696 (Print) Linking ISSN: 08958696 NLM ISO Abbreviation: J Mol Neurosci Subsets: MEDLINE
أسماء مطبوعة: Publication: Totowa, NJ : Humana Press
Original Publication: Boston : Birkhäuser [i.e. Cambridge, MA : Birkhäuser Boston, c1989-
مواضيع طبية MeSH: Gene Expression Regulation, Enzymologic*/drug effects , Promoter Regions, Genetic*/drug effects , Transcription, Genetic*/drug effects, Adrenal Medulla/*enzymology , Chromaffin Cells/*enzymology , Muscarine/*pharmacology , Nicotine/*pharmacology , Phenylethanolamine N-Methyltransferase/*genetics, Animals ; Base Sequence ; Binding Sites ; Cattle ; Cell Nucleus/physiology ; Cells, Cultured ; Dopamine beta-Hydroxylase/genetics ; Genes, Reporter ; Membrane Potentials/drug effects ; Molecular Sequence Data ; Nuclear Proteins/metabolism ; Potassium/pharmacology ; Transcription Factors/metabolism ; Transfection ; Tyrosine 3-Monooxygenase/genetics
مستخلص: Expression of the gene encoding the epinephrine-synthesizing enzyme phenylethanolamine N-methyltransferase (PNMT) is regulated by hormonal and neural stimuli. Because the 5'-upstream regions of the PNMT do not contain sequences analogous to those demonstrated to convey neural regulation to the tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH) catecholamine-synthesizing enzyme genes, functional and biochemical analyses have been utilized to characterize PNMT promoter responses to cholinergic and depolarizing agents. In primary cultures of bovine adrenal medullary chromaffin cells, reporter gene expression from transiently transfected 3- and 0.9-kb-containing PNMT promoter constructs is stimulated approximately twofold by nicotine and muscarine. Depolarizing concentrations of K+ produce fourfold increases in expression. These responses are not detected with constructs containing the proximal 0.3-kb promoter, indicating that the regions between -273 and -877 bp convey neural responsiveness for the PNMT gene in bovine chromaffin cells. Electrophoretic mobility shift assays (EMSAs) with oligonucleotides encoding these regions of the PNMT promoter revealed distinctions in migration of nuclear protein complexes formed following treatment of chromaffin cells with nicotine, muscarine, or 50 mM K+. Thus, the PNMT promoter between 0.3 and 0.9 kb contains sequences capable of responding to cholinergic and depolarization stimuli. Moreover, these treatments influence the interactions of specific nuclear proteins with this region of the PNMT promoter.
References: J Biol Chem. 1992 Sep 15;267(26):18821-30. (PMID: 1382062)
Science. 1985 Nov 22;230(4728):912-6. (PMID: 3904002)
Brain Res Mol Brain Res. 1990 Jun;8(1):25-9. (PMID: 1974315)
Brain Res. 1990 Mar 5;510(2):277-88. (PMID: 1970506)
J Neurosci. 1996 Jul 1;16(13):4102-12. (PMID: 8753872)
J Neurochem. 1992 Dec;59(6):2285-96. (PMID: 1359019)
Neurochem Int. 1989;15(4):555-65. (PMID: 20504532)
Biotechniques. 1988 May;6(5):454-8. (PMID: 2908509)
J Biol Chem. 1992 Apr 15;267(11):7563-9. (PMID: 1348505)
J Neurochem. 1998 Jun;70(6):2286-95. (PMID: 9603193)
J Neurochem. 1996 Oct;67(4):1344-51. (PMID: 8858914)
J Biol Chem. 1995 May 12;270(19):11161-7. (PMID: 7744747)
J Biol Chem. 1993 Jul 25;268(21):15689-95. (PMID: 8101843)
J Neurochem. 1997 Jun;68(6):2241-7. (PMID: 9166715)
Nucleic Acids Res. 1983 Mar 11;11(5):1475-89. (PMID: 6828386)
J Mol Neurosci. 1997 Oct;9(2):127-40. (PMID: 9407393)
J Mol Neurosci. 1991;3(2):75-83. (PMID: 1726044)
Brain Res Mol Brain Res. 1990 Jul;8(2):121-7. (PMID: 1976198)
J Biol Chem. 1993 Nov 5;268(31):23704-11. (PMID: 7901211)
Mol Pharmacol. 1991 Aug;40(2):193-202. (PMID: 1678851)
J Biol Chem. 1993 Aug 25;268(24):17987-94. (PMID: 7688735)
J Neurosci. 1994 Apr;14(4):2106-16. (PMID: 7512633)
J Neurochem. 1992 Dec;59(6):2040-7. (PMID: 1359011)
Brain Res Mol Brain Res. 1992 May;13(4):313-9. (PMID: 1320721)
J Neurosci. 1994 Nov;14(11 Pt 2):7200-7. (PMID: 7525897)
J Biol Chem. 1997 Feb 28;272(9):6051-8. (PMID: 9038229)
Proc Natl Acad Sci U S A. 1988 May;85(10):3648-52. (PMID: 2835776)
J Neurosci Res. 1988 Mar;19(3):367-76. (PMID: 3379652)
Brain Res Mol Brain Res. 1994 Mar;22(1-4):309-19. (PMID: 7912405)
Anal Biochem. 1976 May 7;72:248-54. (PMID: 942051)
J Neurosci. 1990 Feb;10(2):520-30. (PMID: 2303857)
J Biol Chem. 1998 Sep 11;273(37):24065-74. (PMID: 9727025)
Gene Anal Tech. 1988 Mar-Apr;5(2):22-31. (PMID: 3192155)
J Neurosci. 1990 Aug;10(8):2825-33. (PMID: 2167356)
Comput Appl Biosci. 1991 Apr;7(2):203-6. (PMID: 2059845)
Cell Mol Neurobiol. 1992 Jun;12(3):193-215. (PMID: 1358447)
J Biol Chem. 1995 Sep 15;270(37):21579-89. (PMID: 7665571)
J Biol Chem. 1995 Jul 21;270(29):17299-305. (PMID: 7615530)
معلومات مُعتمدة: GM 46588 United States GM NIGMS NIH HHS
المشرفين على المادة: 0 (Nuclear Proteins)
0 (Transcription Factors)
6M3C89ZY6R (Nicotine)
7T101UWZ5W (Muscarine)
EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
EC 1.14.17.1 (Dopamine beta-Hydroxylase)
EC 2.1.1.28 (Phenylethanolamine N-Methyltransferase)
RWP5GA015D (Potassium)
تواريخ الأحداث: Date Created: 20000115 Date Completed: 20000203 Latest Revision: 20181113
رمز التحديث: 20221213
DOI: 10.1385/JMN:12:1:53
PMID: 10636470
قاعدة البيانات: MEDLINE
الوصف
تدمد:0895-8696
DOI:10.1385/JMN:12:1:53