دورية أكاديمية
أعرض في EDS

Histone-containing immune complexes are to a large extent responsible for anti-dsDNA reactivity in the Farr assay of active SLE patients.

التفاصيل البيبلوغرافية
العنوان: Histone-containing immune complexes are to a large extent responsible for anti-dsDNA reactivity in the Farr assay of active SLE patients.
المؤلفون: Hylkema MN; Department of Autoimmune Diseases, CLB, Amsterdam, The Netherlands., van Bruggen MC, ten Hove T, de Jong J, Swaak AJ, Berden JH, Smeenk RJ
المصدر: Journal of autoimmunity [J Autoimmun] 2000 Mar; Vol. 14 (2), pp. 159-68.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: England NLM ID: 8812164 Publication Model: Print Cited Medium: Print ISSN: 0896-8411 (Print) Linking ISSN: 08968411 NLM ISO Abbreviation: J Autoimmun Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Academic Press
Original Publication: London ; San Diego : Academic Press, c1988-
مواضيع طبية MeSH: RNA, Small Cytoplasmic*, Antibodies, Antinuclear/*blood , Antigen-Antibody Complex/*blood , Histones/*immunology , Lupus Erythematosus, Systemic/*immunology, Animals ; Antibodies, Antinuclear/analysis ; Antibodies, Viral/analysis ; Antibodies, Viral/blood ; Antibody Affinity ; Antigen-Antibody Complex/analysis ; Autoantigens/immunology ; Enzyme-Linked Immunosorbent Assay ; Herpesvirus 4, Human/immunology ; Humans ; Immunoglobulin G/analysis ; Immunoglobulin G/blood ; Immunoglobulin M/analysis ; Immunoglobulin M/blood ; Mice ; Radioimmunoprecipitation Assay ; Ribonucleoproteins/immunology
مستخلص: Increased titres of anti-dsDNA antibodies, especially if of high avidity, are associated with renal exacerbations in patients with systemic lupus erythematosus (SLE). One of the most reliable assays to measure anti-dsDNA antibodies, the Farr assay, is believed to detect preferentially high avidity antibodies. Purified non-complexed monoclonal antibodies (mAbs) against nucleosomes, obtained from mice with SLE, are not reactive in the Farr assay, but can become so once complexed to nucleosomes. These Farr-positive, nucleosome containing, immune complexes were also able to bind in vivo to the glomerular basement membrane (GBM), predominantly via heparan sulphate (HS). To evaluate whether in SLE patients the same kind of immune complexes are responsible for Farr reactivity, IgG from serum or plasma was isolated under dissociating and physiological conditions. We observed that after purification under dissociating conditions, Farr reactivity was significantly decreased (P<0.0001) in contrast to reactivity with histones and two 'control' antigens: Epstein Barr Virus (EBV) and Ro/SS-A. Reactivity with nucleosomes also decreased after purification, although to a lesser extent. Plasma purified under physiological conditions showed no decrease in Farr reactivity. The importance of histones for the generation of immune complexes is supported by the two following observations. Firstly, the presence of histones could be demonstrated in serum and plasma of SLE patients but not in serum of healthy controls or in IgG preparations purified under dissociating conditions. Secondly, Farr reactivity of purified IgG preparations could be restored by addition of purified histones. From these studies we conclude that histones containing immune complexes are responsible for a large part of the Farr reactivity in active SLE, and are therefore indirectly implicated in the pathogenesis of lupus nephritis.
(Copyright 2000 Academic Press.)
المشرفين على المادة: 0 (Antibodies, Antinuclear)
0 (Antibodies, Viral)
0 (Antigen-Antibody Complex)
0 (Autoantigens)
0 (Histones)
0 (Immunoglobulin G)
0 (Immunoglobulin M)
0 (RNA, Small Cytoplasmic)
0 (RO60 protein, human)
0 (Ribonucleoproteins)
0 (SS-A antigen)
تواريخ الأحداث: Date Created: 20000324 Date Completed: 20000421 Latest Revision: 20201214
رمز التحديث: 20221213
DOI: 10.1006/jaut.1999.0354
PMID: 10677247
قاعدة البيانات: MEDLINE
الوصف
تدمد:0896-8411
DOI:10.1006/jaut.1999.0354