دورية أكاديمية
أعرض في EDS

Modulation of host immune responses, induction of apoptosis and inhibition of NF-kappaB activation by the Bordetella type III secretion system.

التفاصيل البيبلوغرافية
العنوان: Modulation of host immune responses, induction of apoptosis and inhibition of NF-kappaB activation by the Bordetella type III secretion system.
المؤلفون: Yuk MH; Department of Microbiology and Immunology and Molecular Biology Institute, UCLA School of Medicine, Los Angeles, CA 90095-1747, USA., Harvill ET, Cotter PA, Miller JF
المصدر: Molecular microbiology [Mol Microbiol] 2000 Mar; Vol. 35 (5), pp. 991-1004.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.
اللغة: English
بيانات الدورية: Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 8712028 Publication Model: Print Cited Medium: Print ISSN: 0950-382X (Print) Linking ISSN: 0950382X NLM ISO Abbreviation: Mol Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, OX ; Boston, MA : Blackwell Scientific Publications, c1987-
مواضيع طبية MeSH: Apoptosis*, Antibodies, Bacterial/*biosynthesis , Bordetella bronchiseptica/*pathogenicity , NF-kappa B/*antagonists & inhibitors, Animals ; Bacterial Proteins/genetics ; Base Sequence ; Bordetella bronchiseptica/immunology ; Bordetella bronchiseptica/isolation & purification ; DNA Primers ; Epithelial Cells/microbiology ; Female ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, SCID ; NF-kappa B/metabolism ; Respiratory System/microbiology ; Virulence
مستخلص: Bordetella bronchiseptica establishes respiratory tract infections in laboratory animals with high efficiency. Colonization persists for the life of the animal and infection is usually asymptomatic in immunocompetent hosts. We hypothesize that this reflects a balance between immunostimulatory events associated with infection and immunomodulatory events mediated by the bacteria. We have identified 15 loci that are part of a type III secretion apparatus in B. bronchiseptica and three secreted proteins. The functions of the type III secretion system were investigated by comparing the phenotypes of wild-type bacteria with two strains that are defective in type III secretion using in vivo and in vitro infection models. Type III secretion mutants were defective in long-term colonization of the trachea in immunocompetent mice. The mutants also elicited higher titres of anti-Bordetella antibodies upon infection compared with wild-type bacteria. Type III secretion mutants also showed increased lethal virulence in immunodeficient SCID-beige mice. These observations suggest that type III-secreted products of B. bronchiseptica interact with components of both innate and adaptive immune systems of the host. B. bronchiseptica induced apoptosis in macrophages in vitro and inflammatory cells in vivo and type III secretion was required for this process. Infection of an epithelial cell line with high numbers of wild type, but not type III deficient B. bronchiseptica resulted in rapid aggregation of NF-kappaB into large complexes in the cytoplasm. NF-kappaB aggregation was dependent on type III secretion and aggregated NF-kappaB did not respond to TNFalpha activation, suggesting B. bronchiseptica may modulate host immunity by inactivating NF-kappaB. Based on these in vivo and in vitro results, we hypothesize that the Bordetella type III secretion system functions to modulate host immune responses during infection.
معلومات مُعتمدة: AI38417 United States AI NIAID NIH HHS; AI43986 United States AI NIAID NIH HHS
المشرفين على المادة: 0 (Antibodies, Bacterial)
0 (Bacterial Proteins)
0 (DNA Primers)
0 (NF-kappa B)
تواريخ الأحداث: Date Created: 20000311 Date Completed: 20000413 Latest Revision: 20190725
رمز التحديث: 20231215
DOI: 10.1046/j.1365-2958.2000.01785.x
PMID: 10712682
قاعدة البيانات: MEDLINE
الوصف
تدمد:0950-382X
DOI:10.1046/j.1365-2958.2000.01785.x