دورية أكاديمية
أعرض في EDS

Incorporation of polyunsaturated fatty acids into CT-26, a transplantable murine colonic adenocarcinoma.

التفاصيل البيبلوغرافية
العنوان: Incorporation of polyunsaturated fatty acids into CT-26, a transplantable murine colonic adenocarcinoma.
المؤلفون: Gaposchkin DP; The Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Massachusetts 02118, USA., Zoeller RA, Broitman SA
المصدر: Lipids [Lipids] 2000 Feb; Vol. 35 (2), pp. 181-6.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
اللغة: English
بيانات الدورية: Publisher: Wiley Subscription Services, Inc Country of Publication: United States NLM ID: 0060450 Publication Model: Print Cited Medium: Print ISSN: 0024-4201 (Print) Linking ISSN: 00244201 NLM ISO Abbreviation: Lipids Subsets: MEDLINE
أسماء مطبوعة: Publication: 2018- : Hoboken, NJ : Wiley Subscription Services, Inc.
Original Publication: Chicago, American Oil Chemists' Society.
مواضيع طبية MeSH: Adenocarcinoma/*metabolism , Colonic Neoplasms/*metabolism , Fatty Acids, Unsaturated/*metabolism, Animals ; Arachidonic Acid/metabolism ; Arachidonic Acid/pharmacokinetics ; Docosahexaenoic Acids/metabolism ; Docosahexaenoic Acids/pharmacokinetics ; Eicosapentaenoic Acid/metabolism ; Eicosapentaenoic Acid/pharmacokinetics ; Fatty Acids/metabolism ; Fatty Acids, Unsaturated/pharmacokinetics ; Mice ; Phospholipids/metabolism ; Tumor Cells, Cultured
مستخلص: Previous studies in our laboratory have shown that marine oils, with high levels of eicosapentaenoic (EPA, 20:5n-3) and docosahexaenoic acids (DHA, 22:6n-3), inhibit the growth of CT-26, a murine colon carcinoma cell line, when implanted into the colons of male BALB/c mice. An in vitro model was developed to study the incorporation of polyunsaturated fatty acids (PUFA) into CT-26 cells in culture. PUFA-induced changes in the phospholipid fatty acid composition and the affinity with which different fatty acids enter the various phospholipid species and subspecies were examined. We found that supplementation of cultured CT-26 cells with either 50 microM linoleic acid (LIN, 18:2n-6), arachidonic acid (AA, 20:4n-6), EPA, or DHA significantly alters the fatty acid composition of CT-26 cells. Incorporation of these fatty acids resulted in decreased levels of monounsaturated fatty acids, while EPA and DHA also resulted in lower levels of AA. While significant elongation of both AA and EPA occurred, LIN remained relatively unmodified. Incorporation of radiolabeled fatty acids into different phospholipid species varied significantly. LIN was incorporated predominantly into phosphatidylcholine and had a much lower affinity for the ethanolamine phospholipids. DHA had a higher affinity for plasmenylethanolamine (1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamine) than the other fatty acids, while EPA had the highest affinity for phosphatidylethanol-amine (1,2-diacyl-sn-glycero-3-phosphoethanolamine). These results demonstrate that, in vitro, significant differences are seen between the various PUFA in CT-26 cells with respect to metabolism and distribution, and these may help to explain differences observed with respect to their effects on tumor growth and metastasis in the transplantable model.
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معلومات مُعتمدة: CA57684 United States CA NCI NIH HHS; GM 50571 United States GM NIGMS NIH HHS
المشرفين على المادة: 0 (Fatty Acids)
0 (Fatty Acids, Unsaturated)
0 (Phospholipids)
25167-62-8 (Docosahexaenoic Acids)
27YG812J1I (Arachidonic Acid)
AAN7QOV9EA (Eicosapentaenoic Acid)
تواريخ الأحداث: Date Created: 20000411 Date Completed: 20000606 Latest Revision: 20220716
رمز التحديث: 20231215
DOI: 10.1007/BF02664768
PMID: 10757549
قاعدة البيانات: MEDLINE
الوصف
تدمد:0024-4201
DOI:10.1007/BF02664768