دورية أكاديمية
Iron deficiency diminishes gallbladder neuronal nitric oxide synthase.
| العنوان: | Iron deficiency diminishes gallbladder neuronal nitric oxide synthase. |
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| المؤلفون: | Swartz-Basile DA; Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA., Goldblatt MI, Blaser C, Decker PA, Ahrendt SA, Sarna SK, Pitt HA |
| المصدر: | The Journal of surgical research [J Surg Res] 2000 May 01; Vol. 90 (1), pp. 26-31. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0376340 Publication Model: Print Cited Medium: Print ISSN: 0022-4804 (Print) Linking ISSN: 00224804 NLM ISO Abbreviation: J Surg Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Academic Press Original Publication: Philadelphia [etc.] |
| مواضيع طبية MeSH: | Iron Deficiencies*, Gallbladder/*enzymology , Nitric Oxide Synthase/*metabolism, Animals ; Blotting, Western ; Body Weight ; Cholelithiasis/etiology ; Cholelithiasis/metabolism ; Cholesterol/metabolism ; Dogs ; Female ; Gallbladder/drug effects ; Gallbladder/physiology ; Muscle Contraction/drug effects ; Nitric Oxide Synthase Type I ; Sincalide/pharmacology ; Transferrin/metabolism |
| مستخلص: | Background: Iron deficiency has been demonstrated in the prairie dog to result in cholesterol crystal formation and altered biliary motility. Gallbladder filling and emptying are influenced by both inhibitory and excitatory stimuli, with nitric oxide (NO) playing a key role in normal relaxation. Iron is a cofactor for nitric oxide synthase. Therefore, we tested the hypothesis that iron deficiency would result in diminished levels of gallbladder neuronal nitric oxide synthase (nNOS) but would not influence the gallbladder's response to excitatory stimuli. Materials and Methods: Twenty adult female prairie dogs were fed either an iron-supplemented (Fe(+)) (200 ppm) control diet (n = 10) or an iron-deficient (Fe-) (8 ppm) diet (n = 10) for 8 weeks. Fasting gallbladder volume was measured. Gallbladder muscle strips were harvested for response to excitatory stimuli and measurement of nNOS protein levels by Western blotting. Muscle strip response to a spectrum of doses of cholecystokinin, acetylcholine, and electrical field stimuli was determined, and the areas under the response curves were calculated. Results: Gallbladder volume increased in the iron-deficient prairie dogs compared with the iron-supplemented group (1.45 +/- 0.27 mL vs 0.80 +/- 0.13 mL, P < 0.05). Iron deficiency diminished the ratio of gallbladder nNOS to beta-actin protein levels (0.05 +/- 0.01 vs 3.48 +/- 1.02, P < 0.05) but resulted in a normal response to excitatory stimuli. Conclusions: We conclude that diminished gallbladder neuronal nitric oxide synthase contributes to the gallbladder stasis that occurs with iron deficiency. This phenomenon may contribute to the increased incidence of gallstones in premenopausal women. (Copyright 2000 Academic Press.) |
| معلومات مُعتمدة: | R01-DK44279-07 United States DK NIDDK NIH HHS |
| المشرفين على المادة: | 0 (Transferrin) 97C5T2UQ7J (Cholesterol) EC 1.14.13.39 (Nitric Oxide Synthase) EC 1.14.13.39 (Nitric Oxide Synthase Type I) M03GIQ7Z6P (Sincalide) |
| تواريخ الأحداث: | Date Created: 20000427 Date Completed: 20000601 Latest Revision: 20211203 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1006/jsre.2000.5827 |
| PMID: | 10781371 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0022-4804 |
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| DOI: | 10.1006/jsre.2000.5827 |