دورية أكاديمية
Development and characterization of antibodies specific to caspase-3-produced alpha II-spectrin 120 kDa breakdown product: marker for neuronal apoptosis.
| العنوان: | Development and characterization of antibodies specific to caspase-3-produced alpha II-spectrin 120 kDa breakdown product: marker for neuronal apoptosis. |
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| المؤلفون: | Nath R; Laboratory of Neuro-biochemistry, Department of Neuroscience Therapeutics, Ann Arbor, MI 48105, USA. rathna.nath@wl.com, Huggins M, Glantz SB, Morrow JS, McGinnis K, Nadimpalli R, Wanga KK |
| المصدر: | Neurochemistry international [Neurochem Int] 2000 Oct; Vol. 37 (4), pp. 351-61. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 8006959 Publication Model: Print Cited Medium: Print ISSN: 0197-0186 (Print) Linking ISSN: 01970186 NLM ISO Abbreviation: Neurochem Int Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford [Elmsford, N. Y.] Pergamon Press. |
| مواضيع طبية MeSH: | Apoptosis*, Antibodies/*immunology , Biomarkers/*analysis , Carrier Proteins/*immunology , Caspases/*metabolism , Microfilament Proteins/*immunology , Neurons/*cytology, Animals ; Antibody Specificity ; Binding Sites ; Carrier Proteins/analysis ; Carrier Proteins/metabolism ; Caspase 3 ; Cattle ; Cells, Cultured ; Cerebellum/cytology ; Chickens/immunology ; Humans ; Microfilament Proteins/analysis ; Microfilament Proteins/metabolism ; Molecular Weight ; Neuroblastoma ; Peptide Fragments/analysis ; Peptide Fragments/immunology ; Peptide Fragments/metabolism ; Rats ; Tumor Cells, Cultured |
| مستخلص: | Alpha II-spectrin (alpha-fodrin) is a demonstrated endogenous substrate for caspase-3 in neurons undergoing unscheduled apoptotic death. We have previously identified the caspase cleavage site that yields the distinctive 120 kDa spectrin breakdown product (SBDP120) as (DSLD(1478)*SVEAL). Here, by using a synthetic peptide (NH(2)-SVEALC) mimicking the neo-N-terminal of SBDP120 as antigen, we report the development of chicken antibodies that specifically recognize the SBDP120 generated by in vitro caspase-3 digestion of bovine alpha-spectrin on Western blot. These anti-SBDP120 antibodies recognize SBDP120 generated by two apoptotic challenges (staurosporine, EGTA) to human neuroblastoma SH-SY5Y cells. Yet they neither react with intact alpha-spectrin nor its other fragments on Western blots. These anti-SBDP120 work equally well in detecting SBDP120 generated in rat cerebellar granule neurons undergoing potassium withdrawal-induced apoptosis. In immunocytochemical studies, these antibodies also specifically stained apoptotic SH-SY5Y or CGN's undergoing apoptosis in a caspase- inhibitor-sensitive manner. These anti-SBDP120s might become powerful markers for apoptotic neurons in various neurological or neurodegenerative conditions in vivo. |
| المشرفين على المادة: | 0 (Antibodies) 0 (Biomarkers) 0 (Carrier Proteins) 0 (Microfilament Proteins) 0 (Peptide Fragments) 0 (fodrin) EC 3.4.22.- (CASP3 protein, human) EC 3.4.22.- (Casp3 protein, rat) EC 3.4.22.- (Caspase 3) EC 3.4.22.- (Caspases) |
| تواريخ الأحداث: | Date Created: 20000529 Date Completed: 20001002 Latest Revision: 20190831 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/s0197-0186(00)00040-1 |
| PMID: | 10825575 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0197-0186 |
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| DOI: | 10.1016/s0197-0186(00)00040-1 |