دورية أكاديمية
Regulation of hepatic delta-6 desaturase expression and its role in the polyunsaturated fatty acid inhibition of fatty acid synthase gene expression in mice.
| العنوان: | Regulation of hepatic delta-6 desaturase expression and its role in the polyunsaturated fatty acid inhibition of fatty acid synthase gene expression in mice. |
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| المؤلفون: | Nakamura MT; Graduate Program of Nutritional Sciences and the Institute for Cellular and Molecular Biology, The University of Texas, Austin, TX 78712, USA., Cho HP, Clarke SD |
| المصدر: | The Journal of nutrition [J Nutr] 2000 Jun; Vol. 130 (6), pp. 1561-5. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0404243 Publication Model: Print Cited Medium: Print ISSN: 0022-3166 (Print) Linking ISSN: 00223166 NLM ISO Abbreviation: J Nutr Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [New York, NY] : Elsevier Original Publication: 1928-1933 : Springfield, Ill. : C. C. Thomas |
| مواضيع طبية MeSH: | Diet*, 5,8,11,14-Eicosatetraynoic Acid/*pharmacology , Fatty Acid Desaturases/*genetics , Fatty Acid Synthases/*genetics , Fatty Acids, Unsaturated/*pharmacology , Gene Expression Regulation, Enzymologic/*physiology , Liver/*enzymology, 5,8,11,14-Eicosatetraynoic Acid/administration & dosage ; Animals ; Body Weight/drug effects ; Fatty Acid Desaturases/physiology ; Fatty Acids, Unsaturated/administration & dosage ; Linoleoyl-CoA Desaturase ; Liver/drug effects ; Male ; Mice ; Mice, Inbred BALB C ; RNA, Messenger/metabolism |
| مستخلص: | Dietary polyunsaturated fatty acids (PUFA) of the (n-6) and (n-3) families uniquely suppress the expression of lipogenic genes while concomitantly inducing the expression of genes encoding proteins of fatty acid oxidation. Although considerable progress has been made toward understanding the nuclear events affected by PUFA, the intracellular mediator responsible for the regulation of hepatic lipogenic gene expression remains unclear. On the basis of earlier fatty acid composition studies, we hypothesized that the Delta-6 desaturase pathway was essential for the production of the fatty acid regulator of gene expression. To address this hypothesis, male BALB/c mice (n = 8/group) were fed for 5 d a high glucose, fat-free diet (FF) or the FF plus 50 g/kg 18:2(n-6) with and without eicosa-5, 8,11,14-tetraynoic acid (ETYA) (200 mg/kg diet), a putative inhibitor of the Delta-6 desaturase pathway. ETYA had no effect on food intake or weight gain, but it completely prevented 18:2(n-6) from suppressing the hepatic abundance of fatty acid synthase mRNA. ETYA ingestion was associated with a decrease in the hepatic content of 20:4(n-6) and an increase in the amount of 18:2(n-6). The fatty acid composition changes elicited by ETYA were accompanied by a decrease in the enzymatic activity of Delta-6 desaturase. Interestingly, the hepatic abundance of Delta-6 desaturase mRNA was actually induced by ETYA one- to twofold. When the product of Delta-6 desaturase, i.e., 18:3(n-6), was added to the ETYA plus 18:2(n-6) diet, the hepatic content of 20:4(n-6) was normalized. In addition, 18:3(n-6) consumption reduced the level of hepatic Delta-6 desaturase mRNA by 50% and completely prevented the increase in fatty acid synthase mRNA that was associated with ETYA ingestion. Apparently, Delta-6 desaturation is an essential step for the PUFA regulation of the fatty acid synthase gene transcription. Finally, the suppression of Delta-6 desaturase by PUFA and its induction by ETYA suggest that the Delta-6 desaturase gene may be regulated by two different lipid-dependent mechanisms. |
| معلومات مُعتمدة: | DK09723 United States DK NIDDK NIH HHS; DK52573 United States DK NIDDK NIH HHS; DK53872 United States DK NIDDK NIH HHS |
| المشرفين على المادة: | 0 (Fatty Acids, Unsaturated) 0 (RNA, Messenger) 1191-85-1 (5,8,11,14-Eicosatetraynoic Acid) EC 1.14.19.- (Fatty Acid Desaturases) EC 1.14.19.3 (Linoleoyl-CoA Desaturase) EC 2.3.1.85 (Fatty Acid Synthases) |
| تواريخ الأحداث: | Date Created: 20000529 Date Completed: 20000623 Latest Revision: 20180330 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1093/jn/130.6.1561 |
| PMID: | 10827210 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0022-3166 |
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| DOI: | 10.1093/jn/130.6.1561 |