دورية أكاديمية
A bcl-x(S) adenovirus demonstrates therapeutic efficacy in an ascites model of human breast cancer.
| العنوان: | A bcl-x(S) adenovirus demonstrates therapeutic efficacy in an ascites model of human breast cancer. |
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| المؤلفون: | Sumantran VN; Department of Internal Medicine and Comprehensive Cancer Center, Cancer and Geriatrics Center, University of Michigan, Ann Arbor, Michigan, USA., Lee DS, Baker VV, Murray S, Strawderman M, Wicha MS |
| المصدر: | Journal of the Society for Gynecologic Investigation [J Soc Gynecol Investig] 2000 May-Jun; Vol. 7 (3), pp. 184-9. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 9433806 Publication Model: Print Cited Medium: Print ISSN: 1071-5576 (Print) Linking ISSN: 10715576 NLM ISO Abbreviation: J Soc Gynecol Investig Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Elsevier, c1994- |
| مواضيع طبية MeSH: | Adenoviridae* , Disease Models, Animal* , Genetic Therapy*, Ascites/*therapy , Breast Neoplasms/*therapy , Proto-Oncogene Proteins c-bcl-2/*genetics, Animals ; Apoptosis ; Breast Neoplasms/pathology ; Cell Survival ; Female ; Gene Expression ; Genetic Vectors ; Humans ; Mice ; Mice, Nude ; Neoplasm Transplantation ; Peritoneum/pathology ; Proto-Oncogene Proteins c-bcl-2/physiology ; Tumor Cells, Cultured ; bcl-X Protein |
| مستخلص: | Objectives: To determine whether a Bcl-x(S) adenoviral vector has therapeutic potential in an ascites model of human breast cancer in nude mice. Methods: Advanced ascites were developed by injecting mice intraperitoneally (IP) with MDA-MB-231 human breast carcinoma cells. Mice received sequential IP injections of the Bcl-x(S) virus or a control lac-Z adenovirus. A third group of mice received no virus. Tumor burden and survival were monitored. Histopathology and necropsies were performed on mice. Results: A single injection of the Bcl-x(S) adenovirus produced no systemic or local toxicity and no abnormal histopathology in normal mice. However, abdominal organs within these mice were transduced with the Bcl-x(S) vector. Adenoviral gene transduction efficiency in MDA-MB-231 ascites was 36+/-6.40% (n = 3). Percent weight change differences revealed that ascites bearing mice injected three times with the Bcl-x(S) vector showed a statistically significant decrease in tumor burden compared with lac-Z-injected mice (n = 7; P = .012 on days 10-15 after the first injection). Mice injected with the Bcl-x(S) vector had significantly greater survival relative to lac-Z-injected mice (n = 7; P = .0004). Bcl-x(S) protein expression was detected in aspirates of mice injected with the Bcl-x(S) vector but not the lac-Z vector. Necropsies revealed that ascites bearing mice injected with Bcl-x(S) vector lacked carcinoma in the peritoneal cavity compared with control mice. Conclusion: The Bcl-x(S) adenovirus can reduce tumor burden and increase survival in an ascites model of advanced stage breast cancer. |
| معلومات مُعتمدة: | IPO1CA75136-01A1 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (BCL2L1 protein, human) 0 (Bcl2l1 protein, mouse) 0 (Proto-Oncogene Proteins c-bcl-2) 0 (bcl-X Protein) |
| تواريخ الأحداث: | Date Created: 20000624 Date Completed: 20000803 Latest Revision: 20191104 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/s1071-5576(00)00048-4 |
| PMID: | 10865187 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. | تدمد: | 1071-5576 |
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| DOI: | 10.1016/s1071-5576(00)00048-4 |