دورية أكاديمية
أعرض في EDS

Ciliogenesis and left-right axis defects in forkhead factor HFH-4-null mice.

التفاصيل البيبلوغرافية
العنوان: Ciliogenesis and left-right axis defects in forkhead factor HFH-4-null mice.
المؤلفون: Brody SL; Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USA. brodys@msnotes.wustl.edu, Yan XH, Wuerffel MK, Song SK, Shapiro SD
المصدر: American journal of respiratory cell and molecular biology [Am J Respir Cell Mol Biol] 2000 Jul; Vol. 23 (1), pp. 45-51.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
اللغة: English
بيانات الدورية: Publisher: American Thoracic Society Country of Publication: United States NLM ID: 8917225 Publication Model: Print Cited Medium: Print ISSN: 1044-1549 (Print) Linking ISSN: 10441549 NLM ISO Abbreviation: Am J Respir Cell Mol Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2000- : New York, NY : American Thoracic Society
Original Publication: [New York, NY : The Association, [c1989-
مواضيع طبية MeSH: Cilia/*pathology , Respiratory System/*embryology , Respiratory System/*pathology , Situs Inversus/*pathology, Abnormalities, Multiple/genetics ; Abnormalities, Multiple/metabolism ; Abnormalities, Multiple/pathology ; Animals ; Brain/abnormalities ; Centrioles/metabolism ; Centrioles/pathology ; Centrioles/ultrastructure ; Cilia/metabolism ; Cilia/ultrastructure ; Embryonic and Fetal Development ; Epithelial Cells/metabolism ; Epithelial Cells/pathology ; Epithelial Cells/ultrastructure ; Female ; Gene Deletion ; Histocytochemistry ; Magnetic Resonance Imaging ; Male ; Mice ; Mice, Knockout ; Microscopy, Electron ; Microtubules/metabolism ; Microtubules/pathology ; Microtubules/ultrastructure ; Microvilli/metabolism ; Microvilli/pathology ; Microvilli/ultrastructure ; Phenotype ; Respiratory System/metabolism ; Respiratory System/ultrastructure ; Situs Inversus/genetics ; Situs Inversus/metabolism ; Trachea/abnormalities ; Trachea/metabolism ; Trachea/pathology ; Trachea/ultrastructure
مستخلص: Cilia have been classified as sensory or motile types on the basis of functional and structural characteristics; however, factors important for regulation of assembly of different cilia types are not well understood. Hepatocyte nuclear factor-3/forkhead homologue 4 (HFH-4) is a winged helix/forkhead transcription factor expressed in ciliated cells of the respiratory tract, oviduct, and ependyma in late development through adulthood. Targeted deletion of the Hfh4 gene resulted in defective ciliogenesis in airway epithelial cells and randomized left-right asymmetry so that half the mice had situs inversus. In HFH-4-null mice, classic motile type cilia with a 9 + 2 microtubule ultrastructure were absent in epithelial cells, including those in the airways. In other organs, sensory cilia with a 9 + 0 microtubule pattern, such as those on olfactory neuroepithelial cells, were present. Ultrastructural analysis of mutant cells with absent 9 + 2 cilia demonstrated that defective ciliogenesis was due to abnormal centriole migration and/or apical membrane docking, suggesting that HFH-4 functions to direct basal body positioning or anchoring. Evaluation of wild-type embryos at gestational days 7.0 to 7.5 revealed Hfh4 expression in embryonic node cells that have monocilium, consistent with a function for this factor at the node in early determination of left- right axis. Analysis of the node of HFH-4 mutant embryos revealed that, in contrast to absent airway cilia, node cilia were present. These observations indicate that there are independent regulatory pathways for node ciliogenesis compared with 9 + 2 type ciliogenesis in airways, and support a central role for HFH-4 in ciliogenesis and left-right axis formation.
تواريخ الأحداث: Date Created: 20000629 Date Completed: 20000814 Latest Revision: 20061115
رمز التحديث: 20221213
DOI: 10.1165/ajrcmb.23.1.4070
PMID: 10873152
قاعدة البيانات: MEDLINE
الوصف
تدمد:1044-1549
DOI:10.1165/ajrcmb.23.1.4070