دورية أكاديمية
Genomic convergence and suppression of centrosome hyperamplification in primary p53-/- cells in prolonged culture.
| العنوان: | Genomic convergence and suppression of centrosome hyperamplification in primary p53-/- cells in prolonged culture. |
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| المؤلفون: | Chiba S; Department of Cell Biology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0521, USA., Okuda M, Mussman JG, Fukasawa K |
| المصدر: | Experimental cell research [Exp Cell Res] 2000 Aug 01; Vol. 258 (2), pp. 310-21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0373226 Publication Model: Print Cited Medium: Print ISSN: 0014-4827 (Print) Linking ISSN: 00144827 NLM ISO Abbreviation: Exp Cell Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando Fl : Academic Press Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Centrosome/*physiology , Tumor Suppressor Protein p53/*physiology, Animals ; Cells, Cultured ; Epithelial Cells/cytology ; Mice ; Time Factors ; Tumor Suppressor Protein p53/genetics |
| مستخلص: | Chromosome instability, a major property of cancer cells, is believed to promote mutations that establish malignant phenotypes. Centrosome hyperamplification and the consequential increase in the frequency of aberrant mitoses are the major causes of chromosome instability in cancer cells that lack the functional p53 tumor suppressor protein. Here, we examined dynamic changes of chromosome and centrosome behaviors during long-term culturing of primary epithelial cells derived from p53-null mice. The heterogeneity in the number of chromosomes per cell in the early to mid passage cell population diminished in late passage cells, giving rise to distinct subpopulations of cells. Concomitantly, centrosome hyperamplification that was observed at a high frequency in early to mid passage cells was suppressed in late passage cells. These results provide an explanation for the frequent observations that some cancer cell lines and tissues that lack functional p53 show normal centrosome behaviors and altered, yet relatively stable, chromosomes. Moreover, our in vitro findings may provide a model for possible genomic convergence in cultured cells. This may be analogous to the genomic convergence model proposed for in vivo tumor progression in which chromosome instability initially imposed during tumorigenesis becomes suppressed when neoplastic cells have acquired chromosome compositions that promise an optimal growth in a given environment. (Copyright 2000 Academic Press.) |
| المشرفين على المادة: | 0 (Tumor Suppressor Protein p53) |
| تواريخ الأحداث: | Date Created: 20000718 Date Completed: 20000822 Latest Revision: 20061115 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1006/excr.2000.4916 |
| PMID: | 10896782 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0014-4827 |
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| DOI: | 10.1006/excr.2000.4916 |