دورية أكاديمية
Tobacco ribosomal DNA spacer element stimulates amplification and expression of heterologous genes.
| العنوان: | Tobacco ribosomal DNA spacer element stimulates amplification and expression of heterologous genes. |
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| المؤلفون: | Borisjuk N; Biotech Center, Foran Hall, Cook College, Rutgers University, 59 Dudley Rd., New Brunswick, NJ 08901-8520, USA., Borisjuk L, Komarnytsky S, Timeva S, Hemleben V, Gleba Y, Raskin I |
| المصدر: | Nature biotechnology [Nat Biotechnol] 2000 Dec; Vol. 18 (12), pp. 1303-6. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature America Publishing Country of Publication: United States NLM ID: 9604648 Publication Model: Print Cited Medium: Print ISSN: 1087-0156 (Print) Linking ISSN: 10870156 NLM ISO Abbreviation: Nat Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Nature America Publishing Original Publication: New York, NY : Nature Pub. Co., [1996- |
| مواضيع طبية MeSH: | Gene Amplification* , Gene Expression* , Plants, Toxic* , Transgenes*, DNA, Ribosomal Spacer/*genetics , Recombinant Proteins/*biosynthesis , Nicotiana/*genetics, Acetolactate Synthase/genetics ; Acetolactate Synthase/metabolism ; Base Sequence ; Drug Resistance ; Herbicides/pharmacology ; Molecular Sequence Data ; Plants, Genetically Modified ; Recombinant Proteins/genetics ; Transcription, Genetic |
| مستخلص: | Here we show that the cis-acting genetic element aps (amplification-promoting sequence), isolated from the nontranscribed spacer region of tobacco ribosomal DNA (rDNA), increases the level of expression of recombinant proteins. Transgenic tobacco plants, transformed with expression cassettes containing the herbicide-resistant acetolactate synthase (hr-ALS) gene or the green fluorescent protein (GFP) gene fused to the aps sequence, had greater levels of corresponding messenger RNAs (mRNAs) and proteins compared to transformants lacking aps. Analysis of transgenic plants showed that aps increased the copy number and transcription of the adjacent heterologous genes and, in the case of hr-ALS, enhanced the herbicide resistance phenotype. Both the increased transgene copy number and enhanced expression were stably inherited. These data provide the first evidence that the aps sequence can be used for gene amplification in transgenic plants and possibly other multicellular organisms. |
| المشرفين على المادة: | 0 (DNA, Ribosomal Spacer) 0 (Herbicides) 0 (Recombinant Proteins) EC 2.2.1.6 (Acetolactate Synthase) |
| تواريخ الأحداث: | Date Created: 20001202 Date Completed: 20010222 Latest Revision: 20231213 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1038/82430 |
| PMID: | 11101812 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1087-0156 |
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| DOI: | 10.1038/82430 |