دورية أكاديمية
A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
| العنوان: | A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi. |
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| المؤلفون: | Ribeiro-Dos-Santos R; Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Bahia, Brazil. rrsantos@e-net.com.br, Mengel JO, Postol E, Soares RA, Ferreira-Fernandez E, Soares MB, Pontes-De-Carvalho LC |
| المصدر: | Parasite immunology [Parasite Immunol] 2001 Feb; Vol. 23 (2), pp. 93-101. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley Country of Publication: England NLM ID: 7910948 Publication Model: Print Cited Medium: Print ISSN: 0141-9838 (Print) Linking ISSN: 01419838 NLM ISO Abbreviation: Parasite Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford, Wiley |
| مواضيع طبية MeSH: | Trypanosoma cruzi*, CD4-Positive T-Lymphocytes/*immunology , Chagas Disease/*immunology, Animals ; Cell Line ; Chronic Disease ; Coculture Techniques ; Disease Models, Animal ; Graft Rejection ; Heart Transplantation ; Interferon-gamma/analysis ; Interleukin-2/analysis ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Inbred DBA ; Mice, Nude ; Myocarditis/immunology ; Myocardium/cytology ; Myocardium/immunology ; Rabbits ; Rats ; Spleen/immunology |
| مستخلص: | To study the role of autoreactive T cells in the pathogenesis of cardiomyopathy in Chagas' disease, we generated a cell line by repeated in vitro antigenic stimulation of purified splenic CD4+ T lymphocytes from a chronically Trypanosoma cruzi-infected mouse. Cells from this line were confirmed to be CD4+ CD8- and proliferated upon stimulation with soluble heart antigens from different animal species, as well as with T. cruzi antigen, in the presence of syngeneic feeder cells. In vitro antigen stimulation of the cell line produced a Th1 cytokine profile, with high levels of IFNgamma and IL-2 and absence of IL-4, IL-5 and IL-10. The cell line also terminated the beating of fetal heart clusters in vitro when cocultured with irradiated syngeneic normal spleen cells. In situ injection of the cell line into well established heart transplants also induced the cessation of heart beating. Finally, adoptive transfer of the cell line to heart-immunized or T. cruzi-infected BALB/c nude mice caused intense heart inflammation. |
| المشرفين على المادة: | 0 (Interleukin-2) 82115-62-6 (Interferon-gamma) |
| تواريخ الأحداث: | Date Created: 20010310 Date Completed: 20010607 Latest Revision: 20190915 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1046/j.1365-3024.2001.00368.x |
| PMID: | 11240900 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0141-9838 |
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| DOI: | 10.1046/j.1365-3024.2001.00368.x |