دورية أكاديمية
Angiotensin selectively activates a subpopulation of postganglionic sympathetic neurons in mice.
| العنوان: | Angiotensin selectively activates a subpopulation of postganglionic sympathetic neurons in mice. |
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| المؤلفون: | Ma X; Department of Internal Medicine, The Cardiovascular Center, University of Iowa College of Medicine, Iowa City, Iowa, USA. xiuying-ma@uiowa.edu, Chapleau MW, Whiteis CA, Abboud FM, Bielefeldt K |
| المصدر: | Circulation research [Circ Res] 2001 Apr 27; Vol. 88 (8), pp. 787-93. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 0047103 Publication Model: Print Cited Medium: Internet ISSN: 1524-4571 (Electronic) Linking ISSN: 00097330 NLM ISO Abbreviation: Circ Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Baltimore, MD : Lippincott Williams & Wilkins Original Publication: Baltimore, Md. Grune & Stratton. |
| مواضيع طبية MeSH: | Angiotensin II/*pharmacology , Neurons/*drug effects , Sympathetic Fibers, Postganglionic/*drug effects, Animals ; Biological Clocks/drug effects ; Calcium/metabolism ; Calcium/pharmacology ; Calcium Channel Blockers/pharmacology ; Calcium Channels/metabolism ; Cells, Cultured ; Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors ; Dose-Response Relationship, Drug ; Enzyme Inhibitors/pharmacology ; Fluorescent Dyes ; Ganglia, Sympathetic/cytology ; Ganglia, Sympathetic/drug effects ; Ganglia, Sympathetic/metabolism ; Intracellular Fluid/metabolism ; Mice ; Mice, Inbred C57BL ; Neurons/cytology ; Neurons/metabolism ; Potassium Chloride/pharmacology ; Protein Kinase C/antagonists & inhibitors ; Receptor, Angiotensin, Type 1 ; Receptor, Angiotensin, Type 2 ; Receptors, Angiotensin/metabolism ; Sympathetic Fibers, Postganglionic/cytology ; Sympathetic Fibers, Postganglionic/metabolism |
| مستخلص: | Angiotensin II (Ang II) increases renal sympathetic nerve activity in anesthetized mice before and after ganglionic blockade, suggesting that Ang II may directly activate postganglionic sympathetic neurons. The present study directly tested this hypothesis in vitro. Neurons were dissociated from aortic-renal and celiac ganglia of C57BL/6J mice. Cytosolic Ca(2+) concentration ([Ca(2+)](i)) was measured with ratio imaging using fura 2. Ang II increased [Ca(2+)](i) in a subpopulation of sympathetic neurons. At a concentration of 200 nmol/L, 14 (67%) of 21 neurons responded with a rise in [Ca(2+)](i). The Ang II type 1 (AT(1)) receptor blocker (losartan, 2 micromol/L) but not the Ang II type 2 (AT(2)) receptor blocker (PD123,319, 4 micromol/L) blocked this effect. The Ang II-induced [Ca(2+)](i) increase was abolished by removal of extracellular Ca(2+) but not altered by depletion of intracellular Ca(2+) stores with thapsigargin. Ang II no longer elicited a [Ca(2+)](i) increase in the presence of lanthanum (25 micromol/L). The specific N-type and L-type Ca(2+) channel blockers, omega-conotoxin GVIA and nifedipine, respectively, significantly inhibited the Ang II-induced [Ca(2+)](i) increase. The protein kinase C inhibitor H7 but not the protein kinase A inhibitor H89 blocked the response to Ang II. These results demonstrate that Ang II selectively activates a subpopulation of postganglionic sympathetic neurons in aortic-renal and celiac ganglia, triggering Ca(2+) influx through voltage-gated Ca(2+) channels. This effect is mediated through AT(1) receptors and requires the activation of protein kinase C. The activation of a subgroup of sympathetic neurons by Ang II may exert unique effects on kidney function in pathological states associated with elevated Ang II. |
| المشرفين على المادة: | 0 (Calcium Channel Blockers) 0 (Calcium Channels) 0 (Enzyme Inhibitors) 0 (Fluorescent Dyes) 0 (Receptor, Angiotensin, Type 1) 0 (Receptor, Angiotensin, Type 2) 0 (Receptors, Angiotensin) 11128-99-7 (Angiotensin II) 660YQ98I10 (Potassium Chloride) EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases) EC 2.7.11.13 (Protein Kinase C) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20010428 Date Completed: 20010719 Latest Revision: 20190706 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1161/hh0801.089542 |
| PMID: | 11325870 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1524-4571 |
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| DOI: | 10.1161/hh0801.089542 |