دورية أكاديمية
Bcl-2 overexpression protects human keratinocyte cells from Ukrain-induced apoptosis but not from G2/M arrest.
| العنوان: | Bcl-2 overexpression protects human keratinocyte cells from Ukrain-induced apoptosis but not from G2/M arrest. |
|---|---|
| المؤلفون: | Roublevskaia IN; Department of Dermatology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA., Haake AR, Polevoda BV |
| المصدر: | Drugs under experimental and clinical research [Drugs Exp Clin Res] 2000; Vol. 26 (5-6), pp. 149-56. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bioscience Ediprint Country of Publication: Switzerland NLM ID: 7802135 Publication Model: Print Cited Medium: Print ISSN: 0378-6501 (Print) Linking ISSN: 03786501 NLM ISO Abbreviation: Drugs Exp Clin Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Geneva : Bioscience Ediprint Original Publication: Barcelona, Prous. |
| مواضيع طبية MeSH: | Alkaloids/*pharmacology , Antineoplastic Agents, Phytogenic/*pharmacology , Apoptosis/*drug effects , Keratinocytes/*drug effects , Keratinocytes/*metabolism , Proto-Oncogene Proteins c-bcl-2/*biosynthesis, Berberine Alkaloids ; Blotting, Western ; Cell Cycle/drug effects ; Cell Line ; Flow Cytometry ; Gene Expression Regulation/drug effects ; Humans ; Mitosis/drug effects ; Phenanthridines ; Proto-Oncogene Proteins c-bcl-2/genetics |
| مستخلص: | Exposure of ME180 and A431 carcinoma cells to Ukrain (NSC-631570), a semisynthetic compound consisting of alkaloids isolated from Chelidonium majus L. (Papaveracea), results in cell cycle arrest at the G2/M phase. Ukrain selectively inhibits growth of ME180 and A431 cells at a concentration range from 3.5 microM to 7.0 microM and induces apoptosis. In contrast, normal human keratinocytes showed no difference in the kinetics of progression through the cell cycle in response to this compound. We found that at a concentration of 7.0 microM of this drug Bcl-2 protein overexpression protected HaCaT cell line keratinocytes against apoptosis induced by Ukrain but did not prevent G2/M arrest. Following exposure of normal keratinocytes to Ukrain, we detected an increase in Bcl-2 protein levels and a significant change in protein modification as suggested by observation of its different isoform with shifted electrophoretic mobility. Bcl-2 protein expression and its isoform distribution did not change substantially in ME180 and A431 carcinoma cells. We also suggest that drug-induced mitotic arrest and apoptosis represent dual Ukrain action on cell cycle progression machinery and Bcl-2-involved program cell death in the cell. |
| المشرفين على المادة: | 0 (Alkaloids) 0 (Antineoplastic Agents, Phytogenic) 0 (Berberine Alkaloids) 0 (Phenanthridines) 0 (Proto-Oncogene Proteins c-bcl-2) 6251Q9UK1S (ukrain) |
| تواريخ الأحداث: | Date Created: 20010510 Date Completed: 20010830 Latest Revision: 20121115 |
| رمز التحديث: | 20221213 |
| PMID: | 11345021 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0378-6501 |
|---|