دورية أكاديمية
Investigation of cell exudates active in carbon tetrachloride and chloroform degradation.
| العنوان: | Investigation of cell exudates active in carbon tetrachloride and chloroform degradation. |
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| المؤلفون: | Koons BW; Department of Civil Engineering, University of Minnesota, 122 Civil Engineering Building, 500 Pillsbury Drive S.E., Minneapolis, MN 55455-0220, USA., Baeseman JL, Novak PJ |
| المصدر: | Biotechnology and bioengineering [Biotechnol Bioeng] 2001 Jul 05; Vol. 74 (1), pp. 12-7. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley Country of Publication: United States NLM ID: 7502021 Publication Model: Print Cited Medium: Print ISSN: 0006-3592 (Print) Linking ISSN: 00063592 NLM ISO Abbreviation: Biotechnol Bioeng Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2005->: Hoboken, NJ : Wiley Original Publication: New York, Wiley. |
| مواضيع طبية MeSH: | Carbon Tetrachloride/*metabolism , Chloroform/*metabolism , Methanosarcina/*metabolism, Biodegradation, Environmental ; Hydrogen-Ion Concentration ; Oxygen/metabolism ; Particle Size ; Temperature |
| مستخلص: | Contamination of groundwater by chlorinated solvents such as carbon tetrachloride (CCl4) and chloroform (CHCl3) is a widespread problem. The cell exudates from the methanogen Methanosarcina thermophila are active in the degradation of CCl4 and CHCl3. This research was performed to characterize these exudates. Examination of the influence of pH indicated that activity was greater under alkaline conditions. Rapid CCl4 degradation occurred from 35-65 degrees C, with first-order degradation rate coefficients increasing as temperature increased. It was found that proteins were not responsible for CCl4 degradation. The active agents in the cell exudates were <10 kDa in size, with degradation activity present in both 1-10 kDa and <1 kDa size ranges. Upon purification of the <10 kDa size range of the cell exudates on a C(18) chromatography column, 17 fractions (out of 100) degraded >50% of the added CCl4 in 8 h. These 17 fractions were pooled into three samples based on their elution time from the C(18) column. One of these pooled samples contained elevated levels of cobalt, zinc, and iron, at 2, 3, and 13 times the levels measured in similarly fractionated and pooled samples of medium, respectively. The UV-visible spectrum of this pooled sample had an absorption maximum at 560-580 nm, which is similar to the absorption maxima of heme (approximately 550 and 575 nm). The two other pooled samples contained elevated levels of zinc at 11 and 22 times the concentration measured in similarly fractionated and pooled samples of medium, respectively, and also contained very low levels of nickel, cobalt, and iron. This research suggests that the cell exudates from M. thermophila contain porphorinogen-type molecules capable of dechlorination, possibly excreted corrinoids, hemes, and zinc-containing molecules. (Copyright 2001 John Wiley & Sons, Inc.) |
| المشرفين على المادة: | 7V31YC746X (Chloroform) CL2T97X0V0 (Carbon Tetrachloride) S88TT14065 (Oxygen) |
| تواريخ الأحداث: | Date Created: 20010516 Date Completed: 20010927 Latest Revision: 20190921 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/bit.1090 |
| PMID: | 11353406 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0006-3592 |
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| DOI: | 10.1002/bit.1090 |