دورية أكاديمية
In situ reverse transcription to detect the cbbL gene and visualize RuBisCO in chemoautotrophic nitrifying bacteria.
| العنوان: | In situ reverse transcription to detect the cbbL gene and visualize RuBisCO in chemoautotrophic nitrifying bacteria. |
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| المؤلفون: | Sinigalliano CD; Southeast Environmental Research Center, Florida International University, Miami 33199, USA. sinigall@fiu.edu, Kuhn DN, Jones RD, Guerrero MA |
| المصدر: | Letters in applied microbiology [Lett Appl Microbiol] 2001 Jun; Vol. 32 (6), pp. 388-93. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8510094 Publication Model: Print Cited Medium: Print ISSN: 0266-8254 (Print) Linking ISSN: 02668254 NLM ISO Abbreviation: Lett Appl Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : Oxford : Oxford University Press Original Publication: Oxford, UK : Published for the Society for Applied Bacteriology by Blackwell Scientific Publications, [c1985- |
| مواضيع طبية MeSH: | Reverse Transcriptase Polymerase Chain Reaction*, Bacterial Proteins/*analysis , Nitrobacter/*enzymology , Nitrosomonas/*enzymology , Proteobacteria/*enzymology , Ribulose-Bisphosphate Carboxylase/*analysis, Bacterial Proteins/metabolism ; Nitrobacter/genetics ; Nitrobacter/metabolism ; Nitrosomonas/genetics ; Nitrosomonas/metabolism ; Proteobacteria/genetics ; Proteobacteria/metabolism ; Quaternary Ammonium Compounds/metabolism ; RNA, Messenger/analysis ; Ribulose-Bisphosphate Carboxylase/metabolism |
| مستخلص: | Aims: In situ methodologies targeting the cbbL gene were used to visualize cells of nitrifying bacteria. Both procaryotic in situ PCR (IS-PCR) and in situ reverse transcription (ISRT) protocols were employed to determine gene presence and expression, respectively. Methods and Results: Aged-oligotropic seawater samples were inoculated with microbial assemblages containing a mixture of actively growing nitrifying bacteria, starved nitrifying bacteria, and heterotrophic bacteria without cbbL. After the molecular manipulations, we found that while all the nitrifiers (healthy or starved) with the cbbL gene were detected by IS-PCR, only the actively growing autotrophic nitrifiers with detectable levels of carbon fixation and nitrification activity were detected by ISRT analysis. Conclusion: These results show how IS-PCR and ISRT supplement each other, and their potential for the analysis of heterogeneous populations where an assortment of healthy and starved/dormant cells are expected. |
| معلومات مُعتمدة: | GM 08205 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Quaternary Ammonium Compounds) 0 (RNA, Messenger) EC 4.1.1.39 (Ribulose-Bisphosphate Carboxylase) |
| تواريخ الأحداث: | Date Created: 20010620 Date Completed: 20010719 Latest Revision: 20190915 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1046/j.1472-765x.2001.00927.x |
| PMID: | 11412349 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0266-8254 |
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| DOI: | 10.1046/j.1472-765x.2001.00927.x |