دورية أكاديمية
The orphan nuclear receptor small heterodimer partner as a novel coregulator of nuclear factor-kappa b in oxidized low density lipoprotein-treated macrophage cell line RAW 264.7.
| العنوان: | The orphan nuclear receptor small heterodimer partner as a novel coregulator of nuclear factor-kappa b in oxidized low density lipoprotein-treated macrophage cell line RAW 264.7. |
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| المؤلفون: | Kim YS; Center for Ligand and Transcription, Pohang University of Science and Technology, Pohang 790-784, Korea., Han CY, Kim SW, Kim JH, Lee SK, Jung DJ, Park SY, Kang H, Choi HS, Lee JW, Pak YK |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2001 Sep 07; Vol. 276 (36), pp. 33736-40. Date of Electronic Publication: 2001 Jul 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Print ISSN: 0021-9258 (Print) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Lipoproteins, LDL/*metabolism , Macrophages/*metabolism , NF-kappa B/*chemistry , NF-kappa B/*metabolism , Receptors, Cytoplasmic and Nuclear/*chemistry , Transcription Factors/*chemistry, Animals ; Blotting, Northern ; Blotting, Western ; Cell Line ; Cell Nucleus/metabolism ; Constitutive Androstane Receptor ; Dimerization ; Dose-Response Relationship, Drug ; Glutathione Transferase/metabolism ; HeLa Cells ; Humans ; Ligands ; Linoleic Acids/pharmacology ; Mice ; Models, Biological ; Plasmids/metabolism ; Protein Binding ; Protein Structure, Tertiary ; Receptors, Cytoplasmic and Nuclear/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Time Factors ; Transcription Factors/metabolism ; Transcription, Genetic ; Transcriptional Activation ; Transfection ; Two-Hybrid System Techniques |
| مستخلص: | Small heterodimer partner (SHP), specifically expressed in liver and a limited number of other tissues, is an unusual orphan nuclear receptor that lacks the conventional DNA binding domain. In this work, we found that SHP expression is abundant in murine macrophage cell line RAW 264.7 but was suppressed by oxidized low density lipoprotein (oxLDL) and its constituent 13-hydroxyoctadecadienoic acid, a ligand for peroxisome proliferator-activated receptor gamma. Furthermore, SHP acted as a transcription coactivator of nuclear factor-kappa B (NF kappa B) and was essential for the previously described NF kappa B transactivation by palmitoyl lysophosphatidylcholine, one of the oxLDL constituents. Accordingly NF kappa B, which was transcriptionally active in the beginning, became progressively inert in oxLDL-treated RAW 264.7 cells as oxLDL decreased the SHP expression. Thus, SHP appears to be an important modulatory component to regulate the transcriptional activities of NF kappa B in oxLDL-treated, resting macrophage cells. |
| المشرفين على المادة: | 0 (Constitutive Androstane Receptor) 0 (Ligands) 0 (Linoleic Acids) 0 (Lipoproteins, LDL) 0 (NF-kappa B) 0 (Receptors, Cytoplasmic and Nuclear) 0 (Transcription Factors) 5204-88-6 (13-hydroxy-9,11-octadecadienoic acid) EC 2.5.1.18 (Glutathione Transferase) |
| تواريخ الأحداث: | Date Created: 20010713 Date Completed: 20011011 Latest Revision: 20211203 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1074/jbc.M101977200 |
| PMID: | 11448950 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0021-9258 |
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| DOI: | 10.1074/jbc.M101977200 |