دورية أكاديمية
Coexpression, copurification, crystallization and preliminary X-ray analysis of a complex of ARL2-GTP and PDE delta.
| العنوان: | Coexpression, copurification, crystallization and preliminary X-ray analysis of a complex of ARL2-GTP and PDE delta. |
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| المؤلفون: | Renault L; Max-Planck-Institut für Molekulare Physiologie, Abteilung Strukturelle Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany., Hanzal-Bayer M, Hillig RC |
| المصدر: | Acta crystallographica. Section D, Biological crystallography [Acta Crystallogr D Biol Crystallogr] 2001 Aug; Vol. 57 (Pt 8), pp. 1167-70. Date of Electronic Publication: 2001 Jul 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 9305878 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0907-4449 (Print) Linking ISSN: 09074449 NLM ISO Abbreviation: Acta Crystallogr D Biol Crystallogr Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Malden, MA : Wiley-Blackwell Original Publication: Copenhagen : Published for the International Union of Crystallography by Munksgaard, c1993- |
| مواضيع طبية MeSH: | 3',5'-Cyclic-GMP Phosphodiesterases/*chemistry , Eye Proteins/*chemistry , GTP-Binding Proteins/*chemistry , Guanosine Triphosphate/*chemistry, 3',5'-Cyclic-GMP Phosphodiesterases/biosynthesis ; 3',5'-Cyclic-GMP Phosphodiesterases/genetics ; Animals ; Crystallization ; Crystallography, X-Ray ; Cyclic Nucleotide Phosphodiesterases, Type 6 ; Escherichia coli ; Eye Proteins/biosynthesis ; Eye Proteins/genetics ; GTP-Binding Proteins/biosynthesis ; GTP-Binding Proteins/genetics ; Humans ; Mice ; Protein Conformation |
| مستخلص: | The small GTPase ARL2 (from Mus musculus) and an effector protein, the delta subunit of human cGMP phosphodiesterase (hPDE delta), were coexpressed and copurified from Escherichia coli as a stable complex. Coexpression significantly increased the otherwise low yield of PDE delta production in E. coli. The complex, which contains ARL2 in the activated GTP-bound form, was crystallized in two forms. The first belongs to the monoclinic space group P2(1), with unit-cell parameters a = 48.1, b = 45.7, c = 74.7 A, beta = 94.0 degrees and one complex (39 kDa) in the asymmetric unit. Cryocooled crystals diffract to 2.3 A using synchrotron radiation. The micro-focused X-ray beam at beamline ID13 (ESRF) allowed the use of very small crystals, which helped to overcome twinning and enabled the identification of a molecular-replacement solution. The second form recrystallized from the first one after several months. These crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 44.5, b = 65.4, c = 104.4 A and one complex in the asymmetric unit. They diffracted to 1.8 A using synchrotron radiation. |
| المشرفين على المادة: | 0 (Eye Proteins) 86-01-1 (Guanosine Triphosphate) EC 3.1.4.35 (3',5'-Cyclic-GMP Phosphodiesterases) EC 3.1.4.35 (Cyclic Nucleotide Phosphodiesterases, Type 6) EC 3.1.4.35 (PDE6B protein, human) EC 3.1.4.35 (Pde6b protein, mouse) EC 3.6.1.- (ARL2 protein, human) EC 3.6.1.- (Arl2 protein, mouse) EC 3.6.1.- (GTP-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20010727 Date Completed: 20011004 Latest Revision: 20190605 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1107/s0907444901009556 |
| PMID: | 11468408 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0907-4449 |
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| DOI: | 10.1107/s0907444901009556 |