دورية أكاديمية
Structural analysis of the polysaccharide from the lipopolysaccharide of Porphyromonas gingivalis strain W50.
| العنوان: | Structural analysis of the polysaccharide from the lipopolysaccharide of Porphyromonas gingivalis strain W50. |
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| المؤلفون: | Paramonov N; MRC Molecular Pathogenesis Group, Department of Medical Microbiology, St. Bartholomew's and The Royal London School of Medicine and Dentistry, Queen Mary College, London, UK., Bailey D, Rangarajan M, Hashim A, Kelly G, Curtis MA, Hounsell EF |
| المصدر: | European journal of biochemistry [Eur J Biochem] 2001 Sep; Vol. 268 (17), pp. 4698-707. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Country of Publication: England NLM ID: 0107600 Publication Model: Print Cited Medium: Print ISSN: 0014-2956 (Print) Linking ISSN: 00142956 NLM ISO Abbreviation: Eur J Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: -2004: Oxford, UK : Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies Original Publication: Berlin, New York, Springer. |
| مواضيع طبية MeSH: | Glucans/*chemistry , Lipopolysaccharides/*chemistry , Porphyromonas gingivalis/*chemistry, Carbohydrate Conformation ; Glucans/metabolism ; Magnetic Resonance Spectroscopy |
| مستخلص: | The lipopolysaccharide (LPS) of Porphyromonas gingivalis is an important pro-inflammatory molecule in periodontal disease and a significant target of the host's specific immune response. In addition, we recently demonstrated using monoclonal antibodies that the Arg-gingipains of P. gingivalis are post-translationally modified with glycan chains that are immunologically related to an LPS preparation from this organism. In the present investigation, we determined the structure of the O-polysaccharide of P. gingivalis W50 that was fully characterized on the basis of 1D and 2D NMR (DQF-COSY, TOCSY, NOESY, ROESY, 1H-13C HSQC and 1H-31P HXTOCSY) and GC-MS data. These data allowed us to conclude that the O-polysaccharide is built up of the tetrasaccharide repeating sequence: -->6)-alpha-D-Glcp-(1-->4)-alpha-L-Rhap-(1-->3)-beta-D-GalNAc-(1-->3)-alpha-D-Galp-(1--> and carries a monophosphoethanolamine residue at position C-2 of the alpha-rhamnose residue in a nonstoichiometric (approximately 60%) amount. These data indicate that the O-polysaccharide of P. gingivalis LPS is composed of an unusually modified tetrasaccharide repeating unit. |
| المشرفين على المادة: | 0 (Glucans) 0 (Lipopolysaccharides) |
| تواريخ الأحداث: | Date Created: 20010905 Date Completed: 20011004 Latest Revision: 20190620 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1046/j.1432-1327.2001.02397.x |
| PMID: | 11532006 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0014-2956 |
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| DOI: | 10.1046/j.1432-1327.2001.02397.x |