دورية أكاديمية
Molecular and functional characterization of the stress-induced protein (SIP) gene and its two transcripts generated by alternative splicing. SIP induced by stress and promotes cell death.
| العنوان: | Molecular and functional characterization of the stress-induced protein (SIP) gene and its two transcripts generated by alternative splicing. SIP induced by stress and promotes cell death. |
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| المؤلفون: | Tomasini R; Centre de Recherche INSERM EMI 0116, 163 Av. de Luminy B. P. 172, 13276 Marseille, France., Samir AA, Vaccaro MI, Pebusque MJ, Dagorn JC, Iovanna JL, Dusetti NJ |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2001 Nov 23; Vol. 276 (47), pp. 44185-92. Date of Electronic Publication: 2001 Sep 13. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Print ISSN: 0021-9258 (Print) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Alternative Splicing* , Heat-Shock Proteins*, Carrier Proteins/*physiology, 3T3 Cells ; Amino Acid Sequence ; Animals ; Apoptosis/physiology ; Apoptosis Regulatory Proteins ; Base Sequence ; Carrier Proteins/genetics ; Cloning, Molecular ; In Situ Hybridization, Fluorescence/methods ; Mice ; Molecular Sequence Data ; RNA, Messenger/genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid |
| مستخلص: | We have used a quantitative fluorescent cDNA microarray hybridization approach to identify pancreatic genes induced by the cellular stress promoted by acute pancreatitis in the mouse. We report the cloning and characterization of one of them that encodes the stress-induced proteins (SIP). The mouse SIP gene is organized into five exons and expands over approximately 20 kilobase pairs. Exon 4 (38 base pairs) is alternatively spliced to generate two transcripts. Northern blot and in situ hybridization showed that both SIP mRNAs are rapidly and strongly induced in acinar cells of the pancreas with acute pancreatitis. They are also constitutively expressed in several other tissues, although with different ratios. They encode proteins of 18 and 27 kDa (SIP(18) and SIP(27)). SIP(27) is identical to the thymus-expressed acidic protein (TEAP) protein, formerly described as a thymus-specific protein. Expression of the SIP(18) and SIP(27)/EGFP or V5 fusion proteins showed that both are nuclear factors. We monitored SIP expression in NIH3T3 cells submitted to various stress agents. UV stress, base damaging, mutagenic stress, ethanol, heat shock, and oxidative stress induced the concomitant expression of SIP(18) and SIP(27) mRNAs. Finally, transient transfection of SIP(18) and SIP(27) expression plasmids induced death by apoptosis in COS7 cells as measured by terminal deoxynucleotidyltransferase-mediated dUTP nick end-labeling staining. In conclusion, the SIP gene is an important element of cellular stress response. It is expressed in many tissues and induced by a variety of stress agents affecting many cellular pathways. SIP generates, by alternative splicing, two nuclear proteins that can promote cell death by apoptosis. |
| سلسلة جزيئية: | GENBANK AY034611; AY034612 |
| المشرفين على المادة: | 0 (Apoptosis Regulatory Proteins) 0 (Carrier Proteins) 0 (Heat-Shock Proteins) 0 (RNA, Messenger) 0 (Trp53inp1 protein, mouse) |
| تواريخ الأحداث: | Date Created: 20010915 Date Completed: 20011220 Latest Revision: 20210209 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1074/jbc.M105647200 |
| PMID: | 11557757 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0021-9258 |
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| DOI: | 10.1074/jbc.M105647200 |