دورية أكاديمية
[Inhibition of binding of activated compliment component C4b with its target].
| العنوان: | [Inhibition of binding of activated compliment component C4b with its target]. |
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| عنوان ترانسليتريتد: | Ingibirovanie sviazyvaniia aktivirovannogo C4b-komponenta komplementa s mishen'iu. |
| المؤلفون: | Kozlov LV; Gabrichevskii Research Institute of Epidemiology and Microbiology, Moscow, ul. Admirala Makarova 10, Moscow, 125212 Russia. l.v.kozlov@mtu-net.ru, Lakhtin VM, Skorokhodova TG, Batalova TN, Shoĭbonov BB, D'iakov VL, Guzova VA, Matveevskaia NS |
| المصدر: | Bioorganicheskaia khimiia [Bioorg Khim] 2000 Nov; Vol. 26 (11), pp. 817-24. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Nauka Country of Publication: Russia (Federation) NLM ID: 7804941 Publication Model: Print Cited Medium: Print ISSN: 0132-3423 (Print) Linking ISSN: 01323423 NLM ISO Abbreviation: Bioorg Khim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva, Nauka |
| مواضيع طبية MeSH: | Complement Activation*, Complement C4b/*chemistry , Immunoglobulin G/*chemistry, Epinephrine/chemistry ; Humans ; Immunoglobulin A/chemistry ; Immunoglobulin M/chemistry ; Lectins/chemistry ; Polysaccharides, Bacterial/chemistry ; Protein Binding ; Salicylic Acid/chemistry |
| مستخلص: | The inhibition of covalent binding of the nascent C4b fragment of the human complement component to its natural target, immunoglobulin G, was studied. To this end, an immunoenzyme system was developed. In this ELISA method, the complement was activated on the sorbed IgG molecules and the resulting nascent C4b fragment acylated IgG or interacted with a competitive inhibitor added to the system. The inhibition constants for binding of the nascent C4b to its target were determined for immunoglobulins G1, G2, G3, G4, M, and A1, as well as for ferritin, yeast mannan, capsid polysaccharides of the Neisseria meningitidis A, B, and C serotypes, diphtheria anatoxin, epinephrine, and salicylic acid. On the basis of the experimental data, the immunoglobulin role at the activation stage of the complement regulation cascade, the relationship between the antigen immunogenicity and its ability to interact with C4b, and the direct effect of a number of therapeutic agents on the complement system were discussed. Lectins of various specificities were shown to inhibit the enzymic activation of C4 by the first complement component and the subsequent C4b sorption to its target, which allowed us to suggest that some oligosaccharide fragments of the C1s and C4 molecules are spatially close to the C1s active site and to the thioester bond of C4. |
| المشرفين على المادة: | 0 (Immunoglobulin A) 0 (Immunoglobulin G) 0 (Immunoglobulin M) 0 (Lectins) 0 (Polysaccharides, Bacterial) 80295-50-7 (Complement C4b) O414PZ4LPZ (Salicylic Acid) YKH834O4BH (Epinephrine) |
| تواريخ الأحداث: | Date Created: 20011108 Date Completed: 20011213 Latest Revision: 20131121 |
| رمز التحديث: | 20240627 |
| PMID: | 11696892 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0132-3423 |
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