دورية أكاديمية
Hepatocyte growth factor and macrophage-stimulating protein are upregulated during excisional wound repair in rats.
| العنوان: | Hepatocyte growth factor and macrophage-stimulating protein are upregulated during excisional wound repair in rats. |
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| المؤلفون: | Cowin AJ; Child Health Research Institute and CRC for Tissue Growth and Repair, Women's and Children's Hospital, 72 King William Road, North Adelaide, South Australia, 5006, Australia. allison.cowin@adelaide.edu.au, Kallincos N, Hatzirodos N, Robertson JG, Pickering KJ, Couper J, Belford DA |
| المصدر: | Cell and tissue research [Cell Tissue Res] 2001 Nov; Vol. 306 (2), pp. 239-50. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0417625 Publication Model: Print Cited Medium: Print ISSN: 0302-766X (Print) Linking ISSN: 0302766X NLM ISO Abbreviation: Cell Tissue Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin, New York, Springer-Verlag. |
| مواضيع طبية MeSH: | Proto-Oncogene Proteins*, Epidermis/*metabolism , Growth Substances/*metabolism , Hepatocyte Growth Factor/*metabolism , Wound Healing/*physiology, Animals ; Biomarkers ; Epidermal Cells ; Epidermis/injuries ; Growth Substances/genetics ; Hepatocyte Growth Factor/genetics ; In Situ Hybridization ; Male ; Microscopy, Fluorescence ; Proto-Oncogene Proteins c-met/genetics ; Proto-Oncogene Proteins c-met/metabolism ; Rats ; Rats, Sprague-Dawley ; Receptor Protein-Tyrosine Kinases/genetics ; Receptor Protein-Tyrosine Kinases/metabolism ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/metabolism ; Up-Regulation |
| مستخلص: | Hepatocyte growth factor (HGF) and macrophage-stimulating protein (MSP) are structurally related molecules that stimulate epithelial cell proliferation and migration. MSP also acts directly as a chemoattractant for resident macrophages. These activities are integral to the wound repair processes of inflammation, epithelialization and tissue remodelling. To begin to examine the involvement of HGF and MSP in healing of cutaneous wounds we have mapped the temporal expression of these two molecules and their receptors, MET and RON respectively, in adult rat excisional wounds. Four 2x2-cm full-thickness excisional wounds were created on the dorsum of 18 rats, and biopsies were taken through the wounds at 3, 5, 7, 14, 21, and 28 days postwounding. These biopsies were analyzed using immunofluorescent staining and in situ hybridization (ISH). The number of cells staining positively for HGF and MET significantly increased in response to wounding. HGF staining and mRNA peaked at 7 days postwounding whereas MET was upregulated earlier, peaking after 3 days. Both HGF and MET protein were observed in fibroblasts of the dermis and in the newly forming granulation tissue. ISH studies also revealed that fibroblasts at the wound edges and within the newly forming granulation tissue also expressed HGF and c-met mRNA. Immunofluorescent staining revealed both MSP and RON within the wound, with maximum staining occurring between 7 and 21 days for both the ligand and receptor. In addition, MSP co-localized with a small subset of ED1-positive cells (monocytes). In contrast, ED2-positive cells (macrophages) did not co-localize with MSP. Thus, increased expression of HGF, MSP and their receptors MET and RON respectively was observed in response to wounding. Furthermore, MSP co-localization with a subset of monocytes may confirm a role for MSP in the activation of mature macrophages, which may be important in tissue remodelling. |
| المشرفين على المادة: | 0 (Biomarkers) 0 (Growth Substances) 0 (Proto-Oncogene Proteins) 0 (Receptors, Cell Surface) 0 (macrophage stimulating protein) 67256-21-7 (Hepatocyte Growth Factor) EC 2.7.10.1 (Proto-Oncogene Proteins c-met) EC 2.7.10.1 (RON protein) EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases) |
| تواريخ الأحداث: | Date Created: 20011110 Date Completed: 20020122 Latest Revision: 20181130 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1007/s004410100443 |
| PMID: | 11702235 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 0302-766X |
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| DOI: | 10.1007/s004410100443 |