دورية أكاديمية
Comparative sequence analysis of the imprinted Dlk1-Gtl2 locus in three mammalian species reveals highly conserved genomic elements and refines comparison with the Igf2-H19 region.
| العنوان: | Comparative sequence analysis of the imprinted Dlk1-Gtl2 locus in three mammalian species reveals highly conserved genomic elements and refines comparison with the Igf2-H19 region. |
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| المؤلفون: | Paulsen M; University of Cambridge, Department of Anatomy, Cambridge CB2 3DY, United Kingdom., Takada S, Youngson NA, Benchaib M, Charlier C, Segers K, Georges M, Ferguson-Smith AC |
| المصدر: | Genome research [Genome Res] 2001 Dec; Vol. 11 (12), pp. 2085-94. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9518021 Publication Model: Print Cited Medium: Print ISSN: 1088-9051 (Print) Linking ISSN: 10889051 NLM ISO Abbreviation: Genome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c1995- |
| مواضيع طبية MeSH: | Repressor Proteins*, Genetic Markers/*genetics , Genomic Imprinting/*genetics , Membrane Proteins/*genetics , Proteins/*genetics, Animals ; Binding Sites/genetics ; CCCTC-Binding Factor ; Conserved Sequence/genetics ; CpG Islands/genetics ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Humans ; Interspersed Repetitive Sequences/genetics ; Intracellular Signaling Peptides and Proteins ; Mice ; Molecular Sequence Data ; Proteins/metabolism ; RNA, Long Noncoding ; Sheep ; Transcription Factors/genetics ; Transcription Factors/metabolism |
| مستخلص: | The Dlk1-Gtl2 domain on mouse chromosome 12 contains reciprocally imprinted genes with the potential to contribute to our understanding of common features involved in imprinting control. We have sequenced this conserved region in the mouse and sheep and included the human sequence in a three species comparison. This analysis resulted in a precise conservation map and identification of highly conserved sequence elements, some of which we have shown previously to be differentially methylated in the mouse. Additionally, this analysis facilitated identification of a CpG-rich tandem repeat array located approximately 13-15 kb upstream of Gtl2. Furthermore, we have identified a third imprinted transcript that overlaps with the last Dlk1 exon in the mouse. This transcript lacks a conserved open reading frame and is probably generated by cleavage of extended Dlk1 transcripts. Because Dlk1 and Gtl2 share many of the imprinting properties of the well-characterized Igf2-H19 domain, it has been proposed that the two regions may be regulated in the same way. Comparative genomic examination of the two domains indicates that although there are similarities, other features are very different, including the location of conserved CTCF-binding sites, and the level of conservation at regulatory regions. |
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| سلسلة جزيئية: | GENBANK AJ320506 |
| المشرفين على المادة: | 0 (CCCTC-Binding Factor) 0 (CTCF protein, human) 0 (Ctcf protein, mouse) 0 (DNA-Binding Proteins) 0 (Genetic Markers) 0 (Ihit1 protein, mouse) 0 (Intracellular Signaling Peptides and Proteins) 0 (MEG3 non-coding RNA, human) 0 (MEG3 non-coding RNA, mouse) 0 (Membrane Proteins) 0 (Proteins) 0 (RNA, Long Noncoding) 0 (Repressor Proteins) 0 (Transcription Factors) 0 (delta protein) |
| تواريخ الأحداث: | Date Created: 20011204 Date Completed: 20011228 Latest Revision: 20181113 |
| رمز التحديث: | 20240627 |
| مُعرف محوري في PubMed: | PMC311216 |
| DOI: | 10.1101/gr.206901 |
| PMID: | 11731499 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1088-9051 |
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| DOI: | 10.1101/gr.206901 |