دورية أكاديمية
أعرض في EDS

Effects of tissue-preparation-induced callose synthesis on estimates of plasmodesma size exclusion limits.

التفاصيل البيبلوغرافية
العنوان: Effects of tissue-preparation-induced callose synthesis on estimates of plasmodesma size exclusion limits.
المؤلفون: Radford JE; Department of Biological Sciences, Monash University, Melbourne, Victoria, Australia., White RG
المصدر: Protoplasma [Protoplasma] 2001; Vol. 216 (1-2), pp. 47-55.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: Austria NLM ID: 9806853 Publication Model: Print Cited Medium: Print ISSN: 0033-183X (Print) Linking ISSN: 0033183X NLM ISO Abbreviation: Protoplasma Subsets: MEDLINE
أسماء مطبوعة: Publication: <1998->: Wien ; New York : Springer
Original Publication: Leipzig : Verlag von Gebrüder Borntraeger, 1927-
مواضيع طبية MeSH: Glucans/*biosynthesis , Intercellular Junctions/*metabolism , Magnoliopsida/*ultrastructure , Plant Roots/*ultrastructure, Antimetabolites/pharmacology ; Arabidopsis/drug effects ; Arabidopsis/metabolism ; Arabidopsis/ultrastructure ; Biological Transport ; Deoxyglucose/pharmacology ; Fluorescent Dyes/metabolism ; Glucans/metabolism ; Intercellular Junctions/ultrastructure ; Magnoliopsida/drug effects ; Magnoliopsida/metabolism ; Microinjections ; Microscopy, Fluorescence ; Molecular Weight ; Plant Epidermis/metabolism ; Plant Epidermis/ultrastructure ; Plant Roots/metabolism ; Polymers/metabolism
مستخلص: Plasmodesmata are often characterised by their size exclusion limit (SEL), which is the molecular weight of the largest dye, introduced by microinjection, that will move from cell to cell. In this study, we investigated whether commonly used techniques for isolation and manipulation of tissues, and microinjection of fluorescent dyes, affected the SEL, and whether any such effects could be ameliorated by inhibiting callose deposition. We examined young root epidermal cells of Arabidopsis thaliana and staminal hair cells of Tradescantia virginiana, two tissues often used in experiments on symplastic transport. Transport in root tips dissected from the main plant body and in stamen hairs removed from the base of the stamen filament was compared with transport in undissected roots and stamen hairs attached to the base of the filament, respectively. Tissues were microinjected with fluorescent dyes (457 Da to > 3 kDa) with or without prior incubation in the callose deposition inhibitors 2-deoxy-D-glucose or aniline blue fluorochrome. In both tissues, dissection reduced the SEL, which was largely prevented by prior incubation in 2-deoxy-D-glucose but not by incubation in aniline blue fluorochrome. Thus, standard methods for tissue preparation can cause sufficient callose deposition to reduce cell-to-cell transport, and this needs to be considered in studies employing microinjection. Introduction of the dyes by pressure injection rather than iontophoresis decreased the SEL in A. thaliana but increased it in T. virginiana, showing that these two injection techniques do not necessarily give identical results and that plasmodesmata in different tissues may respond differently to similar experimental procedures.
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المشرفين على المادة: 0 (Antimetabolites)
0 (Fluorescent Dyes)
0 (Glucans)
0 (Polymers)
9064-51-1 (callose)
9G2MP84A8W (Deoxyglucose)
تواريخ الأحداث: Date Created: 20011206 Date Completed: 20020212 Latest Revision: 20191105
رمز التحديث: 20221213
DOI: 10.1007/BF02680130
PMID: 11732196
قاعدة البيانات: MEDLINE
الوصف
تدمد:0033-183X
DOI:10.1007/BF02680130