دورية أكاديمية
Flavonoids inhibit genetic toxicity produced by carcinogens in cells expressing CYP1A2 and CYP1A1.
| العنوان: | Flavonoids inhibit genetic toxicity produced by carcinogens in cells expressing CYP1A2 and CYP1A1. |
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| المؤلفون: | Lautraite S; School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK., Musonda AC, Doehmer J, Edwards GO, Chipman JK |
| المصدر: | Mutagenesis [Mutagenesis] 2002 Jan; Vol. 17 (1), pp. 45-53. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8707812 Publication Model: Print Cited Medium: Print ISSN: 0267-8357 (Print) Linking ISSN: 02678357 NLM ISO Abbreviation: Mutagenesis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1995- : Swansea, UK : Oxford University Press Original Publication: Oxford ; Washington, DC : Published for the United Kingdom Environmental Mutagen Society by IRL Press, [1986- |
| مواضيع طبية MeSH: | Anticarcinogenic Agents/*pharmacology , Benzo(a)pyrene/*antagonists & inhibitors , Carcinogens/*antagonists & inhibitors , Cytochrome P-450 CYP1A1/*physiology , Cytochrome P-450 CYP1A2/*physiology , Enzyme Inhibitors/*pharmacology , Flavonoids/*pharmacology , Prodrugs/*toxicity , Quercetin/*pharmacology , Quinolines/*antagonists & inhibitors, 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide/analysis ; Animals ; Apigenin ; Benzo(a)pyrene/toxicity ; Biotransformation/drug effects ; Carcinogens/toxicity ; Cells, Cultured/drug effects ; Comet Assay ; Cricetinae ; Cricetulus ; Cytochrome P-450 CYP1A1/antagonists & inhibitors ; Cytochrome P-450 CYP1A1/genetics ; Cytochrome P-450 CYP1A2/genetics ; Cytochrome P-450 CYP1A2 Inhibitors ; DNA Adducts/analysis ; DNA Damage ; Fibroblasts/drug effects ; Humans ; Liver/chemistry ; Liver/drug effects ; Lung ; Male ; Mutagenicity Tests ; Prodrugs/metabolism ; Quinolines/analysis ; Quinolines/toxicity ; Rats ; Rats, Wistar ; Recombinant Fusion Proteins/antagonists & inhibitors ; Recombinant Fusion Proteins/physiology ; Species Specificity |
| مستخلص: | The effects of the flavonoids quercetin, apigenin and chrysin (10 microM) on the genetic toxicity of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and benzo[a]pyrene (BaP) was investigated at sub-cytotoxic concentrations in Chinese hamster V79 cells expressing human or rat cytochromes P450. In V79 r1A2-NH and V79 h1A1-MZ cells, none of the flavonoids increased DNA strand breaks (SB) (measured by the Comet assay) or produced detectable DNA adducts (measured by 32P-post-labelling). Neither IQ nor BaP produced DNA damage in the absence of expressed CYP1A2 or CYP1A1, respectively. DNA damage measured as SB and DNA adducts was detectable in V79 r1A2-NH cells expressing rat CYP1A2 when treated with IQ (2.5-50 microM) and this was inhibited by quercetin. Likewise, DNA damage (SB and DNA adducts) was elevated in V79 h1A1-MZ cells expressing human CYP1A1 when treated with BaP (0.1-0.5 microM) and this was inhibited by chrysin and apigenin, but not by quercetin. The specificity of CYP1A1 inhibition by chrysin and apigenin and CYP1A2 inhibition by quercetin was confirmed by ethylresorufin O-deethylase assay. |
| المشرفين على المادة: | 0 (Anticarcinogenic Agents) 0 (Carcinogens) 0 (Cytochrome P-450 CYP1A2 Inhibitors) 0 (DNA Adducts) 0 (DNA-(2-amino-3-methylimidazo(4,5-f)quinoline) adduct) 0 (Enzyme Inhibitors) 0 (Flavonoids) 0 (Prodrugs) 0 (Quinolines) 0 (Recombinant Fusion Proteins) 0 (benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA) 3417WMA06D (Benzo(a)pyrene) 3CN01F5ZJ5 (chrysin) 55097-80-8 (7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide) 7V515PI7F6 (Apigenin) 9IKM0I5T1E (Quercetin) EC 1.14.14.1 (Cytochrome P-450 CYP1A1) EC 1.14.14.1 (Cytochrome P-450 CYP1A2) G2Q7M1P33X (2-amino-3,4-dimethylimidazo(4,5-f)quinoline) |
| تواريخ الأحداث: | Date Created: 20011226 Date Completed: 20020520 Latest Revision: 20190513 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/mutage/17.1.45 |
| PMID: | 11752233 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0267-8357 |
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| DOI: | 10.1093/mutage/17.1.45 |