دورية أكاديمية
Migration of human hematopoietic progenitor cells across bone marrow endothelium is regulated by vascular endothelial cadherin.
| العنوان: | Migration of human hematopoietic progenitor cells across bone marrow endothelium is regulated by vascular endothelial cadherin. |
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| المؤلفون: | van Buul JD; Department of Experimental Immunohematology, CLB and Laboratory for Experimental and Clinical Immunology, and Department of Hematology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands., Voermans C, van den Berg V, Anthony EC, Mul FP, van Wetering S, van der Schoot CE, Hordijk PL |
| المصدر: | Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2002 Jan 15; Vol. 168 (2), pp. 588-96. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association of Immunologists Country of Publication: United States NLM ID: 2985117R Publication Model: Print Cited Medium: Print ISSN: 0022-1767 (Print) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Bethesda, MD : American Association of Immunologists Original Publication: Baltimore : Williams & Wilkins, c1950- |
| مواضيع طبية MeSH: | Bone Marrow/*physiology , Cadherins/*physiology , Cell Movement/*physiology , Endothelium, Vascular/*physiology , Hematopoietic Stem Cells/*physiology, Antigens, CD ; Antigens, CD34/biosynthesis ; Cell Adhesion/physiology ; Cell Line, Transformed ; Endothelium/cytology ; Endothelium/physiology ; Endothelium, Vascular/cytology ; Gap Junctions/metabolism ; Gap Junctions/physiology ; Hematopoietic Stem Cells/cytology ; Humans ; Intercellular Adhesion Molecule-1/physiology ; Permeability ; Reactive Oxygen Species/pharmacology ; Vascular Cell Adhesion Molecule-1/physiology ; rho GTP-Binding Proteins/physiology |
| مستخلص: | The success of stem cell transplantation depends on the ability of i.v. infused stem cells to engraft the bone marrow, a process referred to as homing. Efficient homing requires migration of CD34(+) cells across the bone marrow endothelium, most likely through the intercellular junctions. In this study, we show that loss of vascular endothelial (VE)-cadherin-mediated endothelial cell-cell adhesion increases the permeability of monolayers of human bone marrow endothelial cells (HBMECs) and stimulates the transendothelial migration of CD34(+) cells in response to stromal cell-derived factor-1alpha. Stromal cell-derived factor-1alpha-induced migration was dependent on VCAM-1 and ICAM-1, even in the absence of VE-cadherin function. Cross-linking of ICAM-1 to mimic the leukocyte-endothelium interaction induced actin stress fiber formation but did not induce loss of endothelial integrity, whereas cross-linking of VCAM-1 increased the HBMEC permeability and induced gaps in the monolayer. In addition, VCAM-1-mediated gap formation in HBMEC was accompanied by and dependent on the production of reactive oxygen species. These data suggest that modulation of VE-cadherin function directly affects the efficiency of transendothelial migration of CD34(+) cells and that activation of ICAM-1 and, in particular, VCAM-1 plays an important role in this process through reorganization of the endothelial actin cytoskeleton and by modulating the integrity of the bone marrow endothelium through the production of reactive oxygen species. |
| المشرفين على المادة: | 0 (Antigens, CD) 0 (Antigens, CD34) 0 (Cadherins) 0 (Reactive Oxygen Species) 0 (Vascular Cell Adhesion Molecule-1) 0 (cadherin 5) 126547-89-5 (Intercellular Adhesion Molecule-1) EC 3.6.5.2 (rho GTP-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20020105 Date Completed: 20020131 Latest Revision: 20220227 |
| رمز التحديث: | 20240627 |
| DOI: | 10.4049/jimmunol.168.2.588 |
| PMID: | 11777950 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0022-1767 |
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| DOI: | 10.4049/jimmunol.168.2.588 |