دورية أكاديمية
Protein kinase C and calcium/calmodulin-activated protein kinase II (CaMK II) suppress nicotinic acetylcholine receptor gene expression in mammalian muscle. A specific role for CaMK II in activity-dependent gene expression.
| العنوان: | Protein kinase C and calcium/calmodulin-activated protein kinase II (CaMK II) suppress nicotinic acetylcholine receptor gene expression in mammalian muscle. A specific role for CaMK II in activity-dependent gene expression. |
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| المؤلفون: | Macpherson P; Mental Health Research Institute and the Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA., Kostrominova T, Tang H, Goldman D |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2002 May 03; Vol. 277 (18), pp. 15638-46. Date of Electronic Publication: 2002 Feb 27. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Print ISSN: 0021-9258 (Print) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Gene Expression Regulation*/drug effects, Calcium-Calmodulin-Dependent Protein Kinases/*metabolism , Muscle, Skeletal/*physiology , Protein Kinase C/*metabolism , Receptors, Nicotinic/*genetics, Animals ; Calcimycin/pharmacology ; Calcium/physiology ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 ; Cells, Cultured ; Electric Stimulation ; Genetic Vectors ; Myogenin/genetics ; Rats ; Receptors, Nicotinic/drug effects ; Recombinant Proteins/metabolism ; Ryanodine/pharmacology ; Suppression, Genetic ; Tetrodotoxin/pharmacology ; Transfection |
| مستخلص: | Nicotinic acetylcholine receptor (nAChR) gene expression is regulated by both muscle activity and increased intracellular calcium. This regulation is an important developmental event that rids receptors from the extrajunctional region of the developing muscle fiber. In avian muscle, it has been proposed that muscle activity suppresses nAChR gene expression via calcium-activated protein kinase C (PKC)-dependent phosphorylation of the myogenic transcription factor, myogenin. Here, we examined the role that PKC and other kinases play in mediating calcium- and activity-dependent suppression of nAChR genes in rat primary myotubes. We found that although activated PKC could regulate nAChR promoter activity and transiently suppressed both nAChR and myogenin gene expression, it did not appear to be required for calcium- or activity-dependent control of nAChR gene expression in mammalian muscle. Neither depletion of PKC from myotubes nor specific pharmacological inhibition of PKC blocked the suppression of nAChR gene expression produced by calcium or muscle depolarization. In contrast, we provide evidence that calcium/calmodulin-activated protein kinase II participates in mediating the effects of muscle depolarization on nAChR and myogenin gene expression. |
| معلومات مُعتمدة: | P01 AG10821 United States AG NIA NIH HHS; R01 NS25153 United States NS NINDS NIH HHS |
| المشرفين على المادة: | 0 (Myog protein, rat) 0 (Myogenin) 0 (Receptors, Nicotinic) 0 (Recombinant Proteins) 15662-33-6 (Ryanodine) 37H9VM9WZL (Calcimycin) 4368-28-9 (Tetrodotoxin) EC 2.7.11.13 (Protein Kinase C) EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2) EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20020306 Date Completed: 20020702 Latest Revision: 20210209 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1074/jbc.M109864200 |
| PMID: | 11877392 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0021-9258 |
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| DOI: | 10.1074/jbc.M109864200 |