دورية أكاديمية
أعرض في EDS

Effects of sildenafil on erectile activity in mice lacking neuronal or endothelial nitric oxide synthase.

التفاصيل البيبلوغرافية
العنوان: Effects of sildenafil on erectile activity in mice lacking neuronal or endothelial nitric oxide synthase.
المؤلفون: Cashen DE; Department of Pharmacology, Merck Research Laboratories, PO Box 2000, Rahway, New Jersey, NJ 07065, USA., MacIntyre DE, Martin WJ
المصدر: British journal of pharmacology [Br J Pharmacol] 2002 Jul; Vol. 136 (5), pp. 693-700.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: England NLM ID: 7502536 Publication Model: Print Cited Medium: Print ISSN: 0007-1188 (Print) Linking ISSN: 00071188 NLM ISO Abbreviation: Br J Pharmacol Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Wiley
Original Publication: London, Macmillian Journals Ltd.
مواضيع طبية MeSH: Erectile Dysfunction/*enzymology , Nitric Oxide Synthase/*deficiency , Penile Erection/*drug effects , Piperazines/*pharmacology, Animals ; Blood Pressure/drug effects ; Blood Pressure/physiology ; Electric Stimulation/methods ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Nitric Oxide Synthase/antagonists & inhibitors ; Nitric Oxide Synthase/metabolism ; Nitric Oxide Synthase Type I ; Nitric Oxide Synthase Type II ; Nitric Oxide Synthase Type III ; Penile Erection/physiology ; Purines ; Signal Transduction/drug effects ; Signal Transduction/physiology ; Sildenafil Citrate ; Sulfones
مستخلص: 1. Using an in vivo model of erectile activity, the effects of sildenafil were studied in mice lacking neuronal or endothelial nitric oxide synthase (nNOS and eNOS, respectively). 2. Under pentobarbitone anaesthesia, intracavernous pressure (ICP) and mean arterial pressure (MAP) were monitored continuously in wild-type, nNOS-/- and eNOS-/- mice. The magnitude of erectile activity was quantified as the ratio of ICP to MAP. 3. No differences in basal ICP or MAP were observed amongst wild-type, eNOS-/- and nNOS-/- mice. Electrical stimulation of the cavernous nerve (ESCN; 4.0 V, 16 Hz, 1 ms, 30 s) evoked increases in ICP and ICP/MAP as well as penile tumescence. Responses to ESCN were reduced in nNOS-/-, but not in eNOS-/- mice. 4. L-NAME (50 mg kg(-1), i.v.) significantly increased MAP and attenuated erectile responses in both wild-type and eNOS-/- mice. 5. Sildenafil (1 mg kg(-1), i.v.) augmented electrically-evoked erectile activity in a voltage-dependent manner in wild-type mice and facilitated erectile responses in eNOS-/- mice. By contrast, sildenafil failed to augment the diminished erectile responses in mice lacking the nNOS isoform. 5. These data reveal the relative importance of nNOS, compared to eNOS, as the critical NOS isoform in the control of erectile function and illustrate that the nNOS isoform is required for sildenafil-induced facilitation of erectile responses in vivo in mice.
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المشرفين على المادة: 0 (Piperazines)
0 (Purines)
0 (Sulfones)
BW9B0ZE037 (Sildenafil Citrate)
EC 1.14.13.39 (Nitric Oxide Synthase)
EC 1.14.13.39 (Nitric Oxide Synthase Type I)
EC 1.14.13.39 (Nitric Oxide Synthase Type II)
EC 1.14.13.39 (Nitric Oxide Synthase Type III)
EC 1.14.13.39 (Nos1 protein, mouse)
EC 1.14.13.39 (Nos3 protein, mouse)
تواريخ الأحداث: Date Created: 20020628 Date Completed: 20021223 Latest Revision: 20220317
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC1573400
DOI: 10.1038/sj.bjp.0704772
PMID: 12086978
قاعدة البيانات: MEDLINE
الوصف
تدمد:0007-1188
DOI:10.1038/sj.bjp.0704772