دورية أكاديمية
Supersensitivity of the cholinergic muscarinic system in the rat's brain is induced by high concentrations of Cu+2.
| العنوان: | Supersensitivity of the cholinergic muscarinic system in the rat's brain is induced by high concentrations of Cu+2. |
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| المؤلفون: | Gutiérrez-Reyes E; Unidad de Investigaciones Bioquímicas José Antonio Moreno Yánez, Centro de Investigaciones Biomédicas, Decanato de Medicina, Universidad Centroccidental Lisandro Alvarado, Barquisimeto, Venezuela., Castañeda-Perozo D, Papale-Centofanti J, Nello-Pérez C, Pascuzzo-Lima C, Moreno-Yanez J, Bonfante-Cabarcas R |
| المصدر: | Investigacion clinica [Invest Clin] 2002 Jun; Vol. 43 (2), pp. 107-17. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Universidad Del Zulia Country of Publication: Venezuela NLM ID: 0421531 Publication Model: Print Cited Medium: Print ISSN: 0535-5133 (Print) Linking ISSN: 05355133 NLM ISO Abbreviation: Invest Clin Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Maracaibo : Universidad Del Zulia |
| مواضيع طبية MeSH: | Brain Chemistry/*drug effects , Copper Sulfate/*pharmacology , Nerve Tissue Proteins/*drug effects , Receptors, Muscarinic/*drug effects, Animals ; Atropine/pharmacology ; Cerebral Cortex/drug effects ; Cerebral Cortex/metabolism ; Chelating Agents/pharmacology ; Cholinergic Fibers/drug effects ; Cholinergic Fibers/physiology ; Copper Sulfate/administration & dosage ; Corpus Striatum/drug effects ; Corpus Striatum/metabolism ; Dose-Response Relationship, Drug ; Hippocampus/drug effects ; Hippocampus/metabolism ; Male ; Maze Learning/drug effects ; Memory/drug effects ; Motor Activity/drug effects ; Muscarinic Agonists/pharmacology ; Muscarinic Antagonists/pharmacology ; Penicillamine/pharmacology ; Pyridoxine/pharmacology ; Quinuclidinyl Benzilate/pharmacology ; Radioligand Assay ; Rats ; Rats, Sprague-Dawley ; Receptors, Muscarinic/metabolism ; Zinc Sulfate/pharmacology |
| مستخلص: | Transition metals have been described as regulators of receptor's function. here, we studied the effects of chronic administration of Cu2+ or the Cu2+ chelator penicillamine (PA) on the functional and binding properties of the muscarinic receptors (MR) on selected areas of rat's brain. Groups of 10 Sprague-Dawley rats were treated daily, for 45 days with either 1) 1 mg/Kg CuSO4 (Cu2+), 2) 100 mg/Kg PA, or 3) saline solution. Double T-maze and motility cages were used for behavioral testing and the binding assays were performed using [3H]-QNB or [3H]-N-MSCP as MR's ligands. Cu2+ brain levels were measured in the cerebral cortex by atomic absorption spectrophotometer. Results showed that PA treated rats displayed a significant decrease of locomotor's activity (LA) and rearing behavior (RB), but a significant increases in memory efficiency (ME). Cu2+ treated rats displayed diminished RB with no significant changes in LA. Cu2+ treated rats displayed higher MR's density (Bmax) in cortex (C), striatum (S), and hippocampus (H). An increase in Bmax was also observed in PA treated rats, but only in C and S. Finally, Cu2+ tissue concentration was significantly higher in C of both Cu2+ and with PA treated animals. In conclusion, 45 days of Cu2+ or PA treatment induced brain hypercuprosis, which was associated with MR binding supersensitivity; however, change in ME was only observed in PA treated rats suggesting that might be still another factor in these experiments besides Cu2+ (i.e., Zn2+ or PA itself) involved in memory modulation. |
| المشرفين على المادة: | 0 (Chelating Agents) 0 (Muscarinic Agonists) 0 (Muscarinic Antagonists) 0 (Nerve Tissue Proteins) 0 (Receptors, Muscarinic) 0 (quinuclidinyl benzilate receptor) 6581-06-2 (Quinuclidinyl Benzilate) 7733-02-0 (Zinc Sulfate) 7C0697DR9I (Atropine) GNN1DV99GX (Penicillamine) KV2JZ1BI6Z (Pyridoxine) LRX7AJ16DT (Copper Sulfate) |
| تواريخ الأحداث: | Date Created: 20020711 Date Completed: 20020903 Latest Revision: 20151119 |
| رمز التحديث: | 20231215 |
| PMID: | 12108025 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0535-5133 |
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