دورية أكاديمية
Cloning of zebrafish ovarian carbonyl reductase-like 20 beta-hydroxysteroid dehydrogenase and characterization of its spatial and temporal expression.
| العنوان: | Cloning of zebrafish ovarian carbonyl reductase-like 20 beta-hydroxysteroid dehydrogenase and characterization of its spatial and temporal expression. |
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| المؤلفون: | Wang Y; Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, China, Hong Kong., Ge W |
| المصدر: | General and comparative endocrinology [Gen Comp Endocrinol] 2002 Jul; Vol. 127 (3), pp. 209-16. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370735 Publication Model: Print Cited Medium: Print ISSN: 0016-6480 (Print) Linking ISSN: 00166480 NLM ISO Abbreviation: Gen Comp Endocrinol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Cloning, Molecular* , Gene Expression*, Alcohol Oxidoreductases/*genetics , Cortisone Reductase/*genetics , Ovary/*enzymology , Zebrafish/*genetics, Alcohol Oxidoreductases/analysis ; Alcohol Oxidoreductases/chemistry ; Amino Acid Sequence ; Animals ; Base Sequence ; Cortisone Reductase/analysis ; Cortisone Reductase/chemistry ; DNA/chemistry ; Female ; Gills/enzymology ; Kidney/enzymology ; Male ; Molecular Sequence Data ; Ovarian Follicle/enzymology ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology ; Testis/enzymology ; Tissue Distribution |
| مستخلص: | 20 beta-Hydroxysteroid dehydrogenase (20 beta-HSD) is a crucial enzyme that converts 17 alpha-hydroxyprogesterone to 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (DHP), which triggers oocyte maturation in most teleost fish. A full-length cDNA for a carbonyl reductase-like 20 beta-HSD (CR/20 beta-HSD) has been cloned from the zebrafish ovary. Although the zebrafish CR/20 beta-HSD is expressed in all of the tissues tested, it is predominantly expressed in the ovary, testis, kidney, and gill. In the ovary, the enzyme was shown to be expressed in the follicle cells and its expression appeared to be constitutive. No significant difference was noticed in the level of CR/20 beta-HSD expression among follicles of different stages. Furthermore, analysis of the ovarian samples taken at different times before spawning showed no significant change of the enzyme expression. In agreement with these results, treatment of the cultured zebrafish ovarian follicle cells with gonadotropin and activin had little effect on the expression of the enzyme. Taken together, these results point to the possibility that the gonadotropin-induced DHP production and final oocyte maturation in the zebrafish may not involve significant change of CR/20 beta-HSD expression as evidenced in the salmonids, or that there might be other isoforms of 20 beta-HSD whose expression is tightly controlled by endocrine and paracrine factors. |
| المشرفين على المادة: | 9007-49-2 (DNA) EC 1.1.- (Alcohol Oxidoreductases) EC 1.1.1.53 (Cortisone Reductase) |
| تواريخ الأحداث: | Date Created: 20020913 Date Completed: 20030304 Latest Revision: 20190814 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/s0016-6480(02)00041-2 |
| PMID: | 12225761 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0016-6480 |
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| DOI: | 10.1016/s0016-6480(02)00041-2 |