دورية أكاديمية
Role of p38 mitogen-activated protein kinase in lipopolysaccharide-induced expression of inducible nitric oxide synthase in human endothelial cells.
| العنوان: | Role of p38 mitogen-activated protein kinase in lipopolysaccharide-induced expression of inducible nitric oxide synthase in human endothelial cells. |
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| المؤلفون: | Kan WH; Department of Pathophysiology, First Military Medical University, Guangzhou 510515, China., Yan WS, Jiang Y, Wang JZ, Qin QH, Zhao KS |
| المصدر: | Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [Di Yi Jun Yi Da Xue Xue Bao] 2002 May; Vol. 22 (5), pp. 388-92. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Di 1 jun yi da xue Country of Publication: China NLM ID: 9426110 Publication Model: Print Cited Medium: Print ISSN: 1000-2588 (Print) Linking ISSN: 10002588 NLM ISO Abbreviation: Di Yi Jun Yi Da Xue Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Guangzhou Shi Tonghe Zhen : Di 1 jun yi da xue, -2005. |
| مواضيع طبية MeSH: | Endothelium, Vascular/*drug effects , Lipopolysaccharides/*pharmacology , Mitogen-Activated Protein Kinases/*physiology , Nitric Oxide/*biosynthesis , Nitric Oxide Synthase/*genetics, Cell Line ; Dose-Response Relationship, Drug ; Endothelium, Vascular/cytology ; Endothelium, Vascular/metabolism ; Fluorescent Antibody Technique ; Gene Expression Regulation, Enzymologic/drug effects ; Humans ; Imidazoles/pharmacology ; Nitric Oxide Synthase/biosynthesis ; Nitric Oxide Synthase Type II ; Pyridines/pharmacology ; RNA, Messenger/drug effects ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; p38 Mitogen-Activated Protein Kinases |
| مستخلص: | Objective: To understand the role of p38 mitogen-activated protein kinase (p38MAPK) in the expression of inducible nitric oxide synthase (iNOS) and NO production in human endothelial cells under the stimulation by lipopolysaccharide (LPS). Methods: NO level in the supernatant of the cell culture media was measured with Griess method, and iNOS protein and mRNA expressions by the cells were detected with immunofluorescence analysis and reverse transcriptase-polymerase chain reaction (RT-PCR) respectively. Immunoprecipitation assay was employed to examine p38 MAPK activity. Results: It was shown that in comparison with the basal level of iNOS expression in cultured endothelial cells line ECV304, iNOS mRNA and protein expressions were significantly increased in the cells after LPS stimulation. In response to LPS treatment, obvious enhancement of p38 MAPK activity in ECV304 took place after the stimulation, with the peak level occurring at 15 min that maintained for approximately 45 min before gradual decline. When treated with SB203580 [4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl) imidazole], a highly specific inhibitor of p38 MAPK, significant inhibition of LPS-induced iNOS protein and mRNA expressions was observed. Conclusions: p38 MAPK plays an important role in iNOS expression and NO production in ECV304 cells, and, inhibition of the signal transduction pathway may consequently be an effective approach to reduce the production of iNOS and other cytokines for the treatment of septic shock. |
| المشرفين على المادة: | 0 (Imidazoles) 0 (Lipopolysaccharides) 0 (Pyridines) 0 (RNA, Messenger) 31C4KY9ESH (Nitric Oxide) EC 1.14.13.39 (NOS2 protein, human) EC 1.14.13.39 (Nitric Oxide Synthase) EC 1.14.13.39 (Nitric Oxide Synthase Type II) EC 2.7.11.24 (Mitogen-Activated Protein Kinases) EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) OU13V1EYWQ (SB 203580) |
| تواريخ الأحداث: | Date Created: 20021023 Date Completed: 20021126 Latest Revision: 20161124 |
| رمز التحديث: | 20231215 |
| PMID: | 12390692 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1000-2588 |
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