دورية أكاديمية
[Construction and expression of red fluorescent protein fusion vector incorporating p38 mitogen-activated protein kinase].
| العنوان: | [Construction and expression of red fluorescent protein fusion vector incorporating p38 mitogen-activated protein kinase]. |
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| المؤلفون: | Gong XW; Department of Pathophysiology and Key Laboratory of Shock and Microcirculation of PLA, First Military Medical University, Guangzhou 510515, China., Qin QH, Wang JZ, Jiang Y |
| المصدر: | Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [Di Yi Jun Yi Da Xue Xue Bao] 2002 Feb; Vol. 22 (2), pp. 171-3. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Di 1 jun yi da xue Country of Publication: China NLM ID: 9426110 Publication Model: Print Cited Medium: Print ISSN: 1000-2588 (Print) Linking ISSN: 10002588 NLM ISO Abbreviation: Di Yi Jun Yi Da Xue Xue Bao Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Guangzhou Shi Tonghe Zhen : Di 1 jun yi da xue, -2005. |
| مواضيع طبية MeSH: | Luminescent Proteins/*biosynthesis , Mitogen-Activated Protein Kinases/*biosynthesis, Gene Expression ; Genetic Vectors ; HeLa Cells ; Humans ; Luminescent Proteins/genetics ; Mitogen-Activated Protein Kinases/genetics ; Recombinant Fusion Proteins/biosynthesis ; Transfection ; p38 Mitogen-Activated Protein Kinases ; Red Fluorescent Protein |
| مستخلص: | Objective: To construct the vector that expresses the fusion protein of p38 mitogen-activated protein kinase (MAPK) and red fluorescent protein (RFP) in mammalian cells. Methods: FLAG-tagged p38 MAPK in pcDNA3 vector was subcloned into RFP vector pDsRed1-N1, the construct of which was then transfected into HeLa cells and observed with fluorescence microscope. Results: The recombinant plasmid was verified by enzyme digestion, PCR and sequence analysis, and p38 MAPK-RFP fusion protein was highly expressed in HeLa cells. Fluorescence microscope found the red fluorescence distributed all over the cytoplasm and in the nuclei as well. Conclusion: The expression vector for p38 MAPK-RFP fusion protein is successfully constructed and effective expression of this fusion protein is achieved, which might be instrumental in the study of intracellular localization of p38 MAPK. |
| المشرفين على المادة: | 0 (Luminescent Proteins) 0 (Recombinant Fusion Proteins) EC 2.7.11.24 (Mitogen-Activated Protein Kinases) EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) |
| تواريخ الأحداث: | Date Created: 20021023 Date Completed: 20021104 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| PMID: | 12390821 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1000-2588 |
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