دورية أكاديمية
Generation of a sequence characterized amplified region probe for authentication of crocodilian species.
العنوان: | Generation of a sequence characterized amplified region probe for authentication of crocodilian species. |
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المؤلفون: | Yau FC; Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China., Wong KL, Wang J, But PP, Shaw PC |
المصدر: | The Journal of experimental zoology [J Exp Zool] 2002 Dec 15; Vol. 294 (4), pp. 382-6. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Wiley-Liss [etc.] Country of Publication: United States NLM ID: 0375365 Publication Model: Print Cited Medium: Print ISSN: 0022-104X (Print) Linking ISSN: 0022104X NLM ISO Abbreviation: J Exp Zool Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [New York, etc.] Wiley-Liss [etc.] |
مواضيع طبية MeSH: | Alligators and Crocodiles/*classification , Alligators and Crocodiles/*genetics , DNA Probes/*genetics , Random Amplified Polymorphic DNA Technique/*methods, Animals ; Base Sequence ; Commerce ; Ecology ; Electrophoresis, Agar Gel ; Molecular Sequence Data ; RNA, Ribosomal, 16S/genetics ; Sensitivity and Specificity ; Species Specificity |
مستخلص: | A 209-base pair (bp) crocodilian-specific sequence characterized amplified region (SCAR) was identified from a 425-bp randomly amplified polymorphic DNA (RAPD) fragment. The 209-bp SCAR was produced from amplifications of DNA extracted from fresh and/or dry meat samples from at least three species of Crocodylus, Caiman crocodylus, and Alligator mississippiensis. No amplification was observed from DNA of other common animal species. The use of SCAR opens the way for quick authentication of crocodilian samples for conservation biology and trade regulation. (Copyright 2002 Wiley-Liss, Inc.) |
المشرفين على المادة: | 0 (DNA Probes) 0 (RNA, Ribosomal, 16S) |
تواريخ الأحداث: | Date Created: 20021204 Date Completed: 20030312 Latest Revision: 20220318 |
رمز التحديث: | 20221213 |
DOI: | 10.1002/jez.10199 |
PMID: | 12461817 |
قاعدة البيانات: | MEDLINE |
تدمد: | 0022-104X |
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DOI: | 10.1002/jez.10199 |