دورية أكاديمية
VEGF release is associated with reduced oxygen tensions in experimental inflammatory arthritis.
| العنوان: | VEGF release is associated with reduced oxygen tensions in experimental inflammatory arthritis. |
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| المؤلفون: | Etherington PJ; Kennedy Institute of Rheumatology Division, Imperial College School of Medicine, London, UK., Winlove P, Taylor P, Paleolog E, Miotla JM |
| المصدر: | Clinical and experimental rheumatology [Clin Exp Rheumatol] 2002 Nov-Dec; Vol. 20 (6), pp. 799-805. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Clinical And Experimental Rheumatology S.A.S Country of Publication: Italy NLM ID: 8308521 Publication Model: Print Cited Medium: Print ISSN: 0392-856X (Print) Linking ISSN: 0392856X NLM ISO Abbreviation: Clin Exp Rheumatol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Pisa : Clinical And Experimental Rheumatology S.A.S Original Publication: Pisa, Italy : Pacini editore, [1983- |
| مواضيع طبية MeSH: | Arthritis, Experimental/*metabolism , Endothelial Growth Factors/*metabolism , Intercellular Signaling Peptides and Proteins/*metabolism , Lymphokines/*metabolism , Oxygen/*metabolism, Animals ; Arthritis, Experimental/etiology ; Arthritis, Experimental/pathology ; Collagen Type II/immunology ; Endothelial Growth Factors/genetics ; Freund's Adjuvant/immunology ; Image Processing, Computer-Assisted ; Intercellular Signaling Peptides and Proteins/genetics ; Joints/metabolism ; Joints/pathology ; Lymphokines/genetics ; Male ; Mice ; Microelectrodes ; Oxygen/analysis ; Oxygen Consumption ; Platelet Endothelial Cell Adhesion Molecule-1/metabolism ; Polarography ; Polymerase Chain Reaction ; RNA, Messenger/metabolism ; Synovial Membrane/metabolism ; Synovial Membrane/pathology ; Vascular Endothelial Growth Factor A ; Vascular Endothelial Growth Factors |
| مستخلص: | Objective: The contribution of local VEGF production and subsequent angiogenesis within the synovial membrane to the propagation of arthritis is unclear. The relationship between synovial oxygenation and blood flow in the development of arthritic disease is unknown. We have therefore measured oxygen levels and perfusion rates in the synovial space in a murine model of arthritis. Methods: Arthritis was induced in DBA/1 mice by immunisation with type II collagen. Oxygen and perfusion levels were measured polarographically using silver needle microelectrodes within the knee joints prior to and 10 days after the onset of arthritis. In addition, synovial cells were isolated from knee joints of naive, pre-arthritic and arthritic mice. Results: Onset of arthritis was associated with a marked reduction in synovial oxygen tensions (pO2). The perfusion rates in naive and arthritic animals were not significantly different: in naive mice, the rate was 0.58 +/- 0.11 ml/min/g and in arthritic joints, 0.64 +/- 0.17 ml/min/g. Furthermore, synovial cells isolated from the knee joints of naive animals did not express mRNA for VEGF, but significant levels were detected in cells from non-arthritic mice immunised with collagen. The onset of arthritis was associated with expression of VEGF mRNA and protein, and correlated negatively with pO2 levels. Conclusion: These data demonstrate that decreases in intra-articular pO2 occur in established arthritic conditions and may be the stimulus for local VEGF production. However, perfusion was not increased in arthritic animals and vascular density was unaltered, suggesting that the neovascularisation associated with inflammatory arthritis, is insufficient to restore oxygen homeostasis in the joint. |
| المشرفين على المادة: | 0 (Collagen Type II) 0 (Endothelial Growth Factors) 0 (Intercellular Signaling Peptides and Proteins) 0 (Lymphokines) 0 (Platelet Endothelial Cell Adhesion Molecule-1) 0 (RNA, Messenger) 0 (Vascular Endothelial Growth Factor A) 0 (Vascular Endothelial Growth Factors) 9007-81-2 (Freund's Adjuvant) S88TT14065 (Oxygen) |
| تواريخ الأحداث: | Date Created: 20030102 Date Completed: 20030530 Latest Revision: 20171116 |
| رمز التحديث: | 20240627 |
| PMID: | 12508771 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0392-856X |
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