دورية أكاديمية
Involvement of Ca2+/calmodulin-dependent protein kinase II in endothelial NO production and endothelium-dependent relaxation.
| العنوان: | Involvement of Ca2+/calmodulin-dependent protein kinase II in endothelial NO production and endothelium-dependent relaxation. |
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| المؤلفون: | Schneider JC; Service de Physiologie-Explorations Fonctionnelles, Centre Hospitalier Universitaire Cochin, Assistance Publique, Hôpitaux de Paris, Université Paris 5, France., El Kebir D, Chéreau C, Lanone S, Huang XL, De Buys Roessingh AS, Mercier JC, Dall'Ava-Santucci J, Dinh-Xuan AT |
| المصدر: | American journal of physiology. Heart and circulatory physiology [Am J Physiol Heart Circ Physiol] 2003 Jun; Vol. 284 (6), pp. H2311-9. Date of Electronic Publication: 2003 Jan 30. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901228 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0363-6135 (Print) Linking ISSN: 03636135 NLM ISO Abbreviation: Am J Physiol Heart Circ Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, Md. : American Physiological Society, |
| مواضيع طبية MeSH: | Calcium-Calmodulin-Dependent Protein Kinases/*metabolism , Endothelium, Vascular/*metabolism , Endothelium, Vascular/*physiology , Muscle, Smooth, Vascular/*physiology , Nitric Oxide/*biosynthesis, Acetylcholine/pharmacology ; Animals ; Aorta, Thoracic/drug effects ; Benzylamines/pharmacology ; Calcimycin/pharmacology ; Calcium/pharmacology ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 ; Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors ; Cell Line ; Cells, Cultured ; Electric Stimulation ; Endothelium, Vascular/enzymology ; Enzyme Inhibitors/pharmacology ; Immunoblotting ; In Vitro Techniques ; Muscle Contraction/physiology ; Muscle Relaxation/physiology ; Muscle, Smooth, Vascular/enzymology ; Nitric Oxide Donors/pharmacology ; Nitric Oxide Synthase/metabolism ; Nitric Oxide Synthase Type III ; Phosphorylation ; Rats ; S-Nitroso-N-Acetylpenicillamine/pharmacology ; Sulfonamides/pharmacology ; Swine ; Thapsigargin/pharmacology ; Vasodilator Agents/pharmacology |
| مستخلص: | Nitric oxide (NO) is synthesized from l-arginine by the Ca(2+)/calmodulin-sensitive endothelial NO synthase (NOS) isoform (eNOS). The present study assesses the role of Ca(2+)/calmodulin-dependent protein kinase II (CaMK II) in endothelium-dependent relaxation and NO synthesis. The effects of three CaMK II inhibitors were investigated in endothelium-intact aortic rings of normotensive rats. NO synthesis was assessed by a NO sensor and chemiluminescence in culture medium of cultured porcine aortic endothelial cells stimulated with the Ca(2+) ionophore A23187 and thapsigargin. Rat aortic endothelial NOS activity was measured by the conversion of l-[(3)H]arginine to l-[(3)H]citrulline. Three CaMK II inhibitors, polypeptide 281-302, KN-93, and lavendustin C, attenuated the endothelium-dependent relaxation of endothelium-intact rat aortic rings in response to acetylcholine, A23187, and thapsigargin. None of the CaMK II inhibitors affected the relaxation induced by NO donors. In a porcine aortic endothelial cell line, KN-93 decreased NO synthesis and caused a rightward shift of the concentration-response curves to A23187 and thapsigargin. In rat aortic endothelial cells, KN-93 significantly decreased bradykinin-induced eNOS activity. These results suggest that CaMK II was involved in NO synthesis as a result of Ca(2+)-dependent activation of eNOS. |
| المشرفين على المادة: | 0 (Benzylamines) 0 (Enzyme Inhibitors) 0 (Nitric Oxide Donors) 0 (Sulfonamides) 0 (Vasodilator Agents) 139298-40-1 (KN 93) 31C4KY9ESH (Nitric Oxide) 37H9VM9WZL (Calcimycin) 67526-95-8 (Thapsigargin) 79032-48-7 (S-Nitroso-N-Acetylpenicillamine) EC 1.14.13.39 (Nitric Oxide Synthase) EC 1.14.13.39 (Nitric Oxide Synthase Type III) EC 1.14.13.39 (Nos3 protein, rat) EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2) EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases) N9YNS0M02X (Acetylcholine) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20030201 Date Completed: 20030620 Latest Revision: 20200930 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1152/ajpheart.00932.2001 |
| PMID: | 12560211 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0363-6135 |
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| DOI: | 10.1152/ajpheart.00932.2001 |