دورية أكاديمية
Minimal residual disease detection in hairy cell leukemia. Comparison of flow cytometric immunophenotyping with clonal analysis using consensus primer polymerase chain reaction for the heavy chain gene.
| العنوان: | Minimal residual disease detection in hairy cell leukemia. Comparison of flow cytometric immunophenotyping with clonal analysis using consensus primer polymerase chain reaction for the heavy chain gene. |
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| المؤلفون: | Sausville JE; Laboratory of Pathology, Bldg 10, Room 2N-108, Mail Stop 1500, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA., Salloum RG, Sorbara L, Kingma DW, Raffeld M, Kreitman RJ, Imus PD, Venzon D, Stetler-Stevenson M |
| المصدر: | American journal of clinical pathology [Am J Clin Pathol] 2003 Feb; Vol. 119 (2), pp. 213-7. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0370470 Publication Model: Print Cited Medium: Print ISSN: 0002-9173 (Print) Linking ISSN: 00029173 NLM ISO Abbreviation: Am J Clin Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2016- : Oxford : Oxford University Press Original Publication: Philadelphia [etc.] Lippincott [etc.] |
| مواضيع طبية MeSH: | Flow Cytometry/*methods , Leukemia, Hairy Cell/*diagnosis , Neoplasm, Residual/*diagnosis , Polymerase Chain Reaction/*methods, Cell Count ; Clone Cells ; Consensus Sequence ; DNA, Neoplasm/genetics ; Gene Rearrangement, B-Lymphocyte, Heavy Chain/genetics ; Humans ; Immunophenotyping ; Leukemia, Hairy Cell/genetics ; Leukemia, Hairy Cell/immunology ; Neoplasm, Residual/genetics ; Neoplasm, Residual/immunology ; Sensitivity and Specificity |
| مستخلص: | We studied 86 specimens from 24 patients with hairy cell leukemia (HCL) to determine the sensitivity of routine flow cytometry (FC) and consensus primer PCR (cpPCR) in this disease. FC was more sensitive, detecting HCL in 48 (56%) of 86 specimens, while clonal B-cell populations were detected by cpPCR in only 23 (27%) of 86 specimens. FC and cpPCR were both more sensitive than morphologic examination. A positive cpPCR result is associated with higher tumor cell numbers than a negative cpPCR result, as determined by FC (P = .0017). We determined cutoff values for number of tumor cells at which cpPCR is consistently positive. At 6.8 tumor cells per microliter, cpPCR would be expected to be positive in at least 90% of the samples. FC was adequate in 86 cases (100%), while cpPCR was adequate in 74 cases (86%). FC is superior to cpPCR for detecting minimal residual HCL. It is more sensitive and more specific and permits quantitation of tumor cell number. |
| المشرفين على المادة: | 0 (DNA, Neoplasm) |
| تواريخ الأحداث: | Date Created: 20030213 Date Completed: 20030226 Latest Revision: 20061115 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1309/G629-9513-NGLC-UB1K |
| PMID: | 12579991 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0002-9173 |
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| DOI: | 10.1309/G629-9513-NGLC-UB1K |