دورية أكاديمية
[Preservation of mucosal specimens before processing in the alkaline single cell microgel electrophoresis assay].
| العنوان: | [Preservation of mucosal specimens before processing in the alkaline single cell microgel electrophoresis assay]. |
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| عنوان ترانسليتريتد: | Lagerung humaner nasaler Mukosa für die Einzelzell-Mikrogelelektrophorese. |
| المؤلفون: | Kleinsasser NH; Klinisch experimentelle Onkologie der Klinik und Poliklinik für Hals-, Nasen- und Ohrenkranke der Ludwig-Maximilians-Universität München. norbert.kleinsasser@klinik.uni-regensburg.de, Kastenbauer ER, Zieger S, Baluschko T, Wallner BC, Harréus UA |
| المصدر: | HNO [HNO] 2003 Feb; Vol. 51 (2), pp. 134-9. |
| نوع المنشور: | Comparative Study; English Abstract; Journal Article |
| اللغة: | German |
| بيانات الدورية: | Publisher: Springer Verlag Country of Publication: Germany NLM ID: 2985099R Publication Model: Print Cited Medium: Print ISSN: 0017-6192 (Print) Linking ISSN: 00176192 NLM ISO Abbreviation: HNO Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Berlin : Springer Verlag Original Publication: Berlin. |
| مواضيع طبية MeSH: | Comet Assay* , Cryopreservation* , Specimen Handling*, Nasal Mucosa/*pathology, Adolescent ; Adult ; Biopsy ; Carcinogenicity Tests ; Carcinogens, Environmental/toxicity ; Cell Survival/drug effects ; Cell Survival/physiology ; DNA Damage ; Environmental Monitoring ; Female ; Humans ; Male ; Middle Aged ; Nasal Mucosa/drug effects ; Tissue and Organ Harvesting |
| مستخلص: | Background: Human mucosal biopsies are established in ecogenotoxicological studies, but up until now they have demanded immediate processing after harvesting. We report our experience with the preservation of specimens either for 24 h at 4 degrees C or for longer periods at -80 degrees C and compare the results with fresh specimens using the alkaline single cell microgel electrophoresis assay. Patients and Methods: Nasal mucosa was harvested from ten patients, transferred to the laboratory and divided into groups for immediate processing,24 h preservation at 4 degrees C and cryopreservation at -80 degrees C. Alkaline single cell microgel electrophoresis assays were performed after separating the specimens into single cells and after exposure to benzo[a]pyrene,benzo[a]pyrene-diolepoxide, N-nitrosodiethylamine, or sodium dichromate. The trypan blue exclusion test was used to assess cytotoxicity. Results: Despite of the fact that cell viability remained stable, after cryopreservation DNA-migration increased significantly for the negative control and benzo[a]pyrene. Although an increase was also seen for sodium dichromate, this was not significant. For benzo[a]pyrene-diolepoxide, N-nitrosodiethylamine and N-methyl-N'-nitro-N-nitrosoguanidine there were no significant changes in DNA-migration. After 24 h in cell medium at 4 degrees C,DNA-migration did not rise compared to the samples which were immediately processed. Conclusions: Preservation of mucosal specimens at 4 degrees C for 24 h may be legitimate in order to facilitate laboratory practice. However, cryopreservation should not be applied because it leads to higher rates of DNA migration in some tested substances in the alkaline single cell microgel electrophoresis assay. |
| المشرفين على المادة: | 0 (Carcinogens, Environmental) |
| تواريخ الأحداث: | Date Created: 20030218 Date Completed: 20030609 Latest Revision: 20091103 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s00106-002-0673-1 |
| PMID: | 12589419 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 0017-6192 |
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| DOI: | 10.1007/s00106-002-0673-1 |