دورية أكاديمية
Non-heme iron protein: a potential target of nitric oxide in acute cardiac allograft rejection.
| العنوان: | Non-heme iron protein: a potential target of nitric oxide in acute cardiac allograft rejection. |
|---|---|
| المؤلفون: | Pieper GM; Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA. gmpieper@mcw.edu, Halligan NL, Hilton G, Konorev EA, Felix CC, Roza AM, Adams MB, Griffith OW |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2003 Mar 18; Vol. 100 (6), pp. 3125-30. Date of Electronic Publication: 2003 Mar 06. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0027-8424 (Print) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Graft Rejection/*metabolism , Heart Transplantation/*physiology , Lysine/*analogs & derivatives , Nitric Oxide/*metabolism , Nonheme Iron Proteins/*metabolism, Aconitate Hydratase/metabolism ; Animals ; Cyclosporine/pharmacology ; Electron Spin Resonance Spectroscopy ; Enzyme Inhibitors/pharmacology ; Graft Rejection/drug therapy ; Graft Rejection/etiology ; Heart Transplantation/adverse effects ; Hemeproteins/chemistry ; Hemeproteins/metabolism ; Immunosuppressive Agents/pharmacology ; Lysine/pharmacology ; Nitric Oxide Synthase/antagonists & inhibitors ; Nitric Oxide Synthase/metabolism ; Nitric Oxide Synthase Type II ; Nonheme Iron Proteins/chemistry ; Rats ; Rats, Inbred Lew ; Rats, Inbred WF ; Rats, Sprague-Dawley ; Transplantation, Homologous ; Transplantation, Isogeneic |
| مستخلص: | We examined iron nitrosylation of non-heme protein and enzymatic activity of the Fe-S cluster protein, aconitase, in acute cardiac allograft rejection. Heterotopic transplantation of donor hearts was performed in histocompatibility matched (isografts: Lewis --> Lewis) and mismatched (allografts: Wistar-Furth --> Lewis) rats. On postoperative days (POD) 4-6, Western blot analysis and immunohistochemistry revealed inducible nitric-oxide synthase (iNOS) protein in allografts but not isografts. EPR spectroscopy revealed background signals at g = 2.003 (for semiquinone) and g = 2.02 and g = 1.94 (for Fe-S cluster protein) in isografts and normal hearts. In contrast, in allografts on POD4, a new axial signal at g = 2.04 and g = 2.02 appeared that was attributed to the dinitrosyl-iron complex formed by nitrosylation of non-heme protein. Appearance of this signal occurred at or before significant nitrosylation of heme protein. Iron nitrosylation of non-heme protein was coincidental with decreases in the nonnitrosylated Fe-S cluster signal at g = 1.94. Aconitase enzyme activity was decreased to approximately 50% of that observed in isograft controls by POD4. Treatment with cyclosporine blocked the (i) elevation of plasma nitrate + nitrite, (ii) up-regulation of iNOS protein, (iii) decrease in Fe-S cluster EPR signal, (iv) formation of dinitrosyl-iron complexes, and (v) loss of aconitase enzyme activity. Formation of dinitrosyl-iron complexes and loss of aconitase activity within allografts also was inhibited by treatment of recipients with a selective iNOS inhibitor, l-N(6)-(1-iminoethyl)lysine. This report shows targeting of an important non-heme Fe-S cluster protein in acute solid organ transplant rejection. |
| References: | Free Radic Biol Med. 2001 Dec 15;31(12):1603-8. (PMID: 11744334) Antioxid Redox Signal. 2001 Feb;3(1):81-8. (PMID: 11291601) Biochim Biophys Acta. 1982 Mar 18;702(1):23-9. (PMID: 6279163) Science. 1983 Aug 19;221(4612):769-70. (PMID: 6308761) J Clin Invest. 1986 Sep;78(3):790-7. (PMID: 3745439) Proc Natl Acad Sci U S A. 1990 Feb;87(3):1223-7. (PMID: 2153975) Biochem Biophys Res Commun. 1990 Jan 15;166(1):119-25. (PMID: 2154196) Biochem J. 1991 Jun 15;276 ( Pt 3):643-8. (PMID: 1648348) J Biol Chem. 1992 Jun 5;267(16):10994-8. (PMID: 1375934) Biochim Biophys Acta. 1992 Jun 29;1135(3):275-9. (PMID: 1320408) J Exp Med. 1993 Aug 1;178(2):643-8. (PMID: 8393479) FASEB J. 1993 Sep;7(12):1124-34. (PMID: 8397130) EMBO J. 1993 Sep;12(9):3643-9. (PMID: 7504626) Biochim Biophys Acta. 1994 May 25;1226(2):225-31. (PMID: 7515690) J Biol Chem. 1994 Nov 25;269(47):29405-8. (PMID: 7961919) J Biol Chem. 1994 Nov 25;269(47):29409-15. (PMID: 7961920) J Exp Med. 1995 Jan 1;181(1):63-70. (PMID: 7528779) Eur J Pharmacol. 1995 Jan 24;273(1-2):15-24. (PMID: 7537678) Transplantation. 1995 Jul 15;60(1):77-82. (PMID: 7624947) Int J Cardiol. 1995 Jul;50(3):243-51. (PMID: 8537148) Transplantation. 1996 Jan 27;61(2):324-8. (PMID: 8600645) Methods Enzymol. 1996;269:37-41. (PMID: 8791635) Methods. 1997 Mar;11(3):319-29. (PMID: 9073575) J Biol Chem. 1997 Aug 15;272(33):20340-7. (PMID: 9252338) Free Radic Biol Med. 1998 Jul 15;25(2):201-7. (PMID: 9667497) Transplantation. 1998 Oct 15;66(7):838-44. (PMID: 9798691) Am J Pathol. 1998 Nov;153(5):1371-6. (PMID: 9811327) J Biol Chem. 1998 Nov 27;273(48):31731-7. (PMID: 9822635) Curr Opin Chem Biol. 1999 Apr;3(2):152-7. (PMID: 10226040) Biochim Biophys Acta. 1999 May 5;1411(2-3):290-309. (PMID: 10320664) Cell Mol Life Sci. 1999 Jul;55(8-9):1003-14. (PMID: 10442085) J Cardiovasc Pharmacol. 2002 Mar;39(3):441-8. (PMID: 11862124) J Cardiovasc Pharmacol. 2000 Jan;35(1):114-20. (PMID: 10630741) Transplantation. 2000 Jan 27;69(2):227-31. (PMID: 10670631) J Biol Chem. 2000 May 12;275(19):14064-9. (PMID: 10799480) Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6550-5. (PMID: 10823926) Kidney Int. 2002 Mar;61(3):839-46. (PMID: 11849435) |
| معلومات مُعتمدة: | R01 HL064637 United States HL NHLBI NIH HHS; DK 48423 United States DK NIDDK NIH HHS; HL 64637 United States HL NHLBI NIH HHS; RR 01008 United States RR NCRR NIH HHS; R01 DK048423 United States DK NIDDK NIH HHS |
| المشرفين على المادة: | 0 (Enzyme Inhibitors) 0 (Hemeproteins) 0 (Immunosuppressive Agents) 0 (N(6)-(1-iminoethyl)lysine) 0 (Nonheme Iron Proteins) 31C4KY9ESH (Nitric Oxide) 83HN0GTJ6D (Cyclosporine) EC 1.14.13.39 (Nitric Oxide Synthase) EC 1.14.13.39 (Nitric Oxide Synthase Type II) EC 1.14.13.39 (Nos2 protein, rat) EC 4.2.1.3 (Aconitate Hydratase) K3Z4F929H6 (Lysine) |
| تواريخ الأحداث: | Date Created: 20030308 Date Completed: 20030421 Latest Revision: 20181113 |
| رمز التحديث: | 20240627 |
| مُعرف محوري في PubMed: | PMC152257 |
| DOI: | 10.1073/pnas.0636938100 |
| PMID: | 12624190 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0027-8424 |
|---|---|
| DOI: | 10.1073/pnas.0636938100 |